Pseudomonas aeruginosa PAO1, PA2323 (gapN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2323
Name
gapN
Synonym: glyceraldehyde-3-phosphate dehydrogenase
Synonym: gapB
Replicon chromosome
Genomic location 2562447 - 2564072 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251013.1
GI 15597519
Affymetrix PA2323_at
Entrez 883046
GenBank AAG05711.1
INSDC AAG05711.1
NCBI Locus Tag PA2323
protein_id(GenBank) gb|AAG05711.1|AE004658_5|gnl|PseudoCAP|PA2323
TIGR NTL03PA02323
UniParc UPI00000C5669
UniProtKB Acc Q9I1F5
UniProtKB ID Q9I1F5_PSEAE
UniRef100 UniRef100_Q9I1F5
UniRef50 UniRef50_Q1Q2M7
UniRef90 UniRef90_Q9I1F5

Product

Feature Type CDS
Coding Frame 1
Product Name
GapN
Product Name Confidence: Class 2
Synonyms triosephosphate dehydrogenase
probable glyceraldehyde-3-phosphate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.21
Kyte-Doolittle Hydrophobicity Value -0.140
Molecular Weight (kDa) 60.0
Isoelectric Point (pI) 6.39

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 596 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003184 (476 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2323
Search term: gapN
Search term: GapN

Human Homologs

Ensembl 86, assembly GRCh38.p7
aldehyde dehydrogenase 3 family member A2 [Source:HGNC Symbol;Acc:HGNC:403]
E-value: 1.0e-16
Percent Identity: 23.5

References

Sequence, expression, and function of the gene for the nonphosphorylating, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Streptococcus mutans.
Boyd DA, Cvitkovitch DG, Hamilton IR
J. Bacteriol. 1995 May;177(10):2622-7
PubMed ID: 7751269
Identification of GntR as regulator of the glucose metabolism in Pseudomonas aeruginosa.
Daddaoua A, Corral-Lugo A, Ramos JL, Krell T.
Environ Microbiol. 2017 Sep;19(9):3721-3733.
PubMed ID: 28752954