Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2386
|
Name |
pvdA
|
Replicon | chromosome |
Genomic location | 2638928 - 2640259 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_251076.1 |
GI | 15597582 |
Affymetrix | PA2386_pvdA_at |
Entrez | 882167 |
GenBank | AAG05774.1 |
INSDC | AAG05774.1 |
NCBI Locus Tag | PA2386 |
protein_id(GenBank) | gb|AAG05774.1|AE004665_1|gnl|PseudoCAP|PA2386 |
TIGR | NTL03PA02386 |
UniParc | UPI0000132B92 |
UniProtKB Acc | Q51548 |
UniProtKB ID | PVDA_PSEAE |
UniRef100 | UniRef100_Q51548 |
UniRef50 | UniRef50_Q51548 |
UniRef90 | UniRef90_Q51548 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
L-ornithine N5-oxygenase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -5.02 |
Kyte-Doolittle Hydrophobicity Value | -0.252 |
Molecular Weight (kDa) | 49.5 |
Isoelectric Point (pI) | 6.46 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3S5W | OXIDOREDUCTASE | 05/23/11 | Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
3S61 | OXIDOREDUCTASE | 05/23/11 | Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 3.03 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | VFDB : VF0094 |
Category | Pyoverdine |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 121 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003141 (460 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2386
Search term: pvdA
Search term: L-ornithine N5-oxygenase
|
Cloning and nucleotide sequence of the pvdA gene encoding the pyoverdin biosynthetic enzyme L-ornithine N5-oxygenase in Pseudomonas aeruginosa.
Visca P, Ciervo A, Orsi N
J Bacteriol 1994 Feb;176(4):1128-40
PubMed ID: 8106324
|
High cellular organization of pyoverdine biosynthesis in Pseudomonas aeruginosa: clustering of PvdA at the old cell pole.
Guillon L, El Mecherki M, Altenburger S, Graumann PL, Schalk IJ
Environ Microbiol 2012 Aug;14(8):1982-94
PubMed ID: 22498339
|
Comprehensive spectroscopic, steady state, and transient kinetic studies of a representative siderophore-associated flavin monooxygenase.
Mayfield JA, Frederick RE, Streit BR, Wencewicz TA, Ballou DP, DuBois JL
J Biol Chem 2010 Oct 1;285(40):30375-88
PubMed ID: 20650894
|
Kinetic mechanism of ornithine hydroxylase (PvdA) from Pseudomonas aeruginosa: substrate triggering of O2 addition but not flavin reduction.
Meneely KM, Barr EW, Bollinger JM Jr, Lamb AL
Biochemistry 2009 May 26;48(20):4371-6
PubMed ID: 19368334
|
Biochemical characterization of a flavin adenine dinucleotide-dependent monooxygenase, ornithine hydroxylase from Pseudomonas aeruginosa, suggests a novel reaction mechanism.
Meneely KM, Lamb AL
Biochemistry 2007 Oct 23;46(42):11930-7
PubMed ID: 17900176
|
Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1.
Vandenende CS, Vlasschaert M, Seah SY
J Bacteriol 2004 Sep;186(17):5596-602
PubMed ID: 15317763
|
Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa.
Imperi F, Putignani L, Tiburzi F, Ambrosi C, Cipollone R, Ascenzi P, Visca P
Microbiology (Reading) 2008 Sep;154(Pt 9):2804-2813
PubMed ID: 18757814
|