Pseudomonas aeruginosa PAO1, PA2386 (pvdA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 2638928 - 2640259 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_251076.1
GI 15597582
Affymetrix PA2386_pvdA_at
Entrez 882167
GenBank AAG05774.1
INSDC AAG05774.1
NCBI Locus Tag PA2386
protein_id(GenBank) gb|AAG05774.1|AE004665_1|gnl|PseudoCAP|PA2386
UniParc UPI0000132B92
UniProtKB Acc Q51548
UniRef100 UniRef100_Q51548
UniRef50 UniRef50_Q51548
UniRef90 UniRef90_Q51548


Feature Type CDS
Coding Frame 1
Product Name
L-ornithine N5-oxygenase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.02
Kyte-Doolittle Hydrophobicity Value -0.252
Molecular Weight (kDa) 49.5
Isoelectric Point (pI) 6.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3S5W OXIDOREDUCTASE 05/23/11 Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
3S61 OXIDOREDUCTASE 05/23/11 Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 3.03 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0094
Category Pyoverdine
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 121 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003141 (476 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2386
Search term: pvdA


Cloning and nucleotide sequence of the pvdA gene encoding the pyoverdin biosynthetic enzyme L-ornithine N5-oxygenase in Pseudomonas aeruginosa.
Visca P, Ciervo A, Orsi N
J. Bacteriol. 1994 Feb;176(4):1128-40
PubMed ID: 8106324
High cellular organization of pyoverdine biosynthesis in Pseudomonas aeruginosa: clustering of PvdA at the old cell pole.
Guillon L, El Mecherki M, Altenburger S, Graumann PL, Schalk IJ
Environ. Microbiol. 2012 Aug;14(8):1982-94
PubMed ID: 22498339
Comprehensive spectroscopic, steady state, and transient kinetic studies of a representative siderophore-associated flavin monooxygenase.
Mayfield JA, Frederick RE, Streit BR, Wencewicz TA, Ballou DP, DuBois JL
J. Biol. Chem. 2010 Oct;285(40):30375-88
PubMed ID: 20650894
Kinetic mechanism of ornithine hydroxylase (PvdA) from Pseudomonas aeruginosa: substrate triggering of O2 addition but not flavin reduction.
Meneely KM, Barr EW, Bollinger JM, Lamb AL
Biochemistry 2009 May;48(20):4371-6
PubMed ID: 19368334
Biochemical characterization of a flavin adenine dinucleotide-dependent monooxygenase, ornithine hydroxylase from Pseudomonas aeruginosa, suggests a novel reaction mechanism.
Meneely KM, Lamb AL
Biochemistry 2007 Oct;46(42):11930-7
PubMed ID: 17900176
Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1.
Vandenende CS, Vlasschaert M, Seah SY
J. Bacteriol. 2004 Sep;186(17):5596-602
PubMed ID: 15317763
Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa.
Imperi F, Putignani L, Tiburzi F, Ambrosi C, Cipollone R, Ascenzi P, Visca P
Microbiology (Reading, Engl.) 2008 Sep;154(Pt 9):2804-13
PubMed ID: 18757814