Pseudomonas aeruginosa PAO1, PA2949

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2949
Name
Replicon chromosome
Genomic location 3308391 - 3309338 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 7 transposon mutants in orthologs

Cross-References

RefSeq NP_251639.1
GI 15598145
Affymetrix PA2949_at
DNASU PaCD00007285
Entrez 882855
GenBank AAG06337.1
INSDC AAG06337.1
NCBI Locus Tag PA2949
protein_id(GenBank) gb|AAG06337.1|AE004721_5|gnl|PseudoCAP|PA2949
TIGR NTL03PA02949
UniParc UPI00000C4EC6
UniProtKB Acc G3XCU7
UniProtKB ID G3XCU7_PSEAE
UniRef100 UniRef100_Q9KJG6
UniRef50 UniRef50_F6AH52
UniRef90 UniRef90_Q9KJG6

Product

Feature Type CDS
Coding Frame 1
Product Name
esterase
Product Name Confidence: Class 3
Synonyms probable lipase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.10
Kyte-Doolittle Hydrophobicity Value -0.029
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6I8W HYDROLASE 11/21/18 Crystal structure of a membrane phospholipase A, a novel bacterial virulence factor Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 503 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000798 (2691 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2949
Search term: esterase

Human Homologs

Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 3 [Source:HGNC Symbol;Acc:HGNC:23760]
E-value: 9.0e-15
Percent Identity: 24.8
Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 3 [Source:HGNC Symbol;Acc:HGNC:23760]
E-value: 9.0e-15
Percent Identity: 24.8
Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 3 [Source:HGNC Symbol;Acc:HGNC:23760]
E-value: 9.0e-15
Percent Identity: 24.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of the lipase gene lip3 from the antarctic psychotroph Moraxella TA144.
Feller G, Thiry M, Gerday C
Biochim Biophys Acta 1991 Feb 16;1088(2):323-4
PubMed ID: 2001407
A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.
Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger KE
FEBS Open Bio 2016 May;6(5):484-93
PubMed ID: 27419054