Pseudomonas aeruginosa PAO1, PA3487 (tle5)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3902663 - 3905962 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_252177.1
GI 15598683
Affymetrix PA3487_at
Entrez 880040
GenBank AAG06875.1
INSDC AAG06875.1
NCBI Locus Tag PA3487
protein_id(GenBank) gb|AAG06875.1|AE004769_8|gnl|PseudoCAP|PA3487
UniParc UPI00000C5A35
UniProtKB Acc Q9HYC2
UniRef100 UniRef100_Q9HYC2
UniRef50 UniRef50_Q9HYC2
UniRef90 UniRef90_Q9HYC2


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Synonyms PldA
Evidence for Translation
Charge (pH 7) -3.85
Kyte-Doolittle Hydrophobicity Value -0.316
Molecular Weight (kDa) 122.3
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Host Associated Class 1
Periplasmic Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB
Category Phospholipase D
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Infection model Rat agar bead model of chronic lung infection
Reference 11136451

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002156 (80 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3487
Search term: tle5
Search term: Tle5

Human Homologs

Ensembl 86, assembly GRCh38.p7
phospholipase D2 [Source:HGNC Symbol;Acc:HGNC:9068]
E-value: 3.1e-25
Percent Identity: 32.3


Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model.
Wilderman PJ, Vasil AI, Johnson Z, Vasil ML
Mol. Microbiol. 2001 Jan;39(2):291-303
PubMed ID: 11136451
Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr;496(7446):508-12
PubMed ID: 23552891
A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells.
Jiang F, Waterfield NR, Yang J, Yang G, Jin Q
Cell Host Microbe 2014 May;15(5):600-10
PubMed ID: 24832454
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
Biochemical characterization of a Pseudomonas aeruginosa phospholipase D.
Spencer C, Brown HA.
Biochemistry. 2015 Feb 10;54(5):1208-18.
PubMed ID: 25565226