Pseudomonas aeruginosa UCBPP-PA14, PA14_01040 (tagJ1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_01040
Name
tagJ1
Synonym: hsiE1
Replicon chromosome
Genomic location 101659 - 102504 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentHcp Secretion Island-I-encoded type VI secretion system (H1-T6SS)

Cross-References

RefSeq YP_788243.1
GI 116053806
Entrez 4383504
INSDC ABJ15042.1
NCBI Locus Tag PA14_01040
UniParc UPI00000C4F27
UniProtKB Acc Q02UZ3
UniProtKB ID Q02UZ3_PSEAB
UniRef100 UniRef100_Q9I746
UniRef50 UniRef50_A1KCF9
UniRef90 UniRef90_Q9I746

Product

Feature Type CDS
Coding Frame 1
Product Name
TagJ1
Product Name Confidence: Class 1
Synonyms secretion protein
Evidence for Translation
Charge (pH 7) -12.54
Kyte-Doolittle Hydrophobicity Value 0.019
Molecular Weight (kDa) 30.4
Isoelectric Point (pI) 4.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4UQZ PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.599 X-RAY DIFFRACTION 100.0
4UQX PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.2 X-RAY DIFFRACTION 100.0
4UQY PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT 1.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 44 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000085 (310 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_01040
Search term: tagJ1
Search term: TagJ1

References

RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.
Förster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A.
J Biol Chem. 2014 Nov 21;289(47):33032-43.
PubMed ID: 25305017
The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system.
Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A.
Mol Microbiol. 2012 Oct;86(2):437-56.
PubMed ID: 22906320