Pseudomonas aeruginosa UCBPP-PA14, PA14_19020 (tse3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_19020
Name
tse3
Replicon chromosome
Genomic location 1642132 - 1643358 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_789663.1
GI 116051502
Entrez 4381468
INSDC ABJ12738.1
NCBI Locus Tag PA14_19020
UniParc UPI00003960CA
UniProtKB Acc Q02QX3
UniProtKB ID Q02QX3_PSEAB
UniRef100 UniRef100_Q02QX3
UniRef50 UniRef50_Q9HYC5
UniRef90 UniRef90_Q9HYC5

Product

Feature Type CDS
Coding Frame 1
Product Name
Tse3
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) 2.97
Kyte-Doolittle Hydrophobicity Value -0.197
Molecular Weight (kDa) 44.4
Isoelectric Point (pI) 9.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4N80 HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 99.7
4M5F HYDROLASE INHIBITOR 08/08/13 complex structure of Tse3-Tsi3 PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 99.8
4LUQ PROTEIN BINDING/TOXIN INHIBITOR 07/25/13 Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3 PSEUDOMONAS AERUGINOSA 1.77 X-RAY DIFFRACTION 99.8
3WA5 HYDROLASE 04/26/13 Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3 PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 99.8
4N7S HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex with Zinc ion PSEUDOMONAS AERUGINOSA 2.101 X-RAY DIFFRACTION 99.8
4M5E HYDROLASE 08/08/13 Tse3 structure PSEUDOMONAS AERUGINOSA 1.49 X-RAY DIFFRACTION 99.8
4N88 HYDROLASE/HYDROLASE INHIBITOR 10/17/13 Crystal structure of Tse3-Tsi3 complex with calcium ion PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002158 (303 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_19020
Search term: tse3
Search term: Tse3

References

RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa.
Lu D, Shang G, Yu Q, Zhang H, Zhao Y, Cang H, Gu L, Xu S, Huang Y.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):524-7.
PubMed ID: 23695568
Structural Insights on the bacteriolytic and self-protection mechanism of muramidase effector Tse3 in Pseudomonas aeruginosa.
Li L, Zhang W, Liu Q, Gao Y, Gao Y, Wang Y, Wang DZ, Li Z, Wang T.
J Biol Chem. 2013 Oct 18;288(42):30607-13.
PubMed ID: 24025333
Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3 complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-binding mechanism.
Lu D, Shang G, Zhang H, Yu Q, Cong X, Yuan J, He F, Zhu C, Zhao Y, Yin K, Chen Y, Hu J, Zhang X, Yuan Z, Xu S, Hu W, Cang H, Gu L.
Mol Microbiol. 2014 Jun;92(5):1092-112.
PubMed ID: 24724564
Complex structure of type VI peptidoglycan muramidase effector and a cognate immunity protein.
Wang T, Ding J, Zhang Y, Wang DC, Liu W.
Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1889-900.
PubMed ID: 24100309