Pseudomonas aeruginosa UCBPP-PA14, PA14_43040 (hsiB2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_43040
Name
hsiB2
Synonym: tssB2
Replicon chromosome
Genomic location 3829090 - 3829596 (- strand)
Transposon Mutants in orthologs 6 transposon mutants in orthologs
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq YP_791589.1
GI 116049606
Entrez 4382919
INSDC ABJ10842.1
NCBI Locus Tag PA14_43040
UniParc UPI00003967E6
UniProtKB Acc Q02KE7
UniProtKB ID Q02KE7_PSEAB
UniRef100 UniRef100_Q02KE7
UniRef50 UniRef50_B6EJ78
UniRef90 UniRef90_W0YNN9

Product

Feature Type CDS
Coding Frame 1
Product Name
HsiB2
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -7.00
Kyte-Doolittle Hydrophobicity Value -0.343
Molecular Weight (kDa) 18.2
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse burn Infection model
Infection model Mouse acute lung Infection model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 107 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000082 (1276 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43040
Search term: hsiB2
Search term: HsiB2

References

Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis.
Lesic B, Starkey M, He J, Hazan R, Rahme LG
Microbiology (Reading, Engl.) 2009 Sep;155(Pt 9):2845-55
PubMed ID: 19497948
The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells.
Sana TG, Hachani A, Bucior I, Soscia C, Garvis S, Termine E, Engel J, Filloux A, Bleves S
J. Biol. Chem. 2012 Aug;287(32):27095-105
PubMed ID: 22665491
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system.
Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A.
Mol Microbiol. 2012 Oct;86(2):437-56.
PubMed ID: 22906320