Pseudomonas aeruginosa UCBPP-PA14, PA14_43050 (hsiA2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_43050
Name
hsiA2
Synonym: tssA2
Replicon chromosome
Genomic location 3829632 - 3831188 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 7 transposon mutants in orthologs
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq YP_791590.1
GI 116049605
Entrez 4381813
INSDC ABJ10841.1
NCBI Locus Tag PA14_43050
UniParc UPI0000E44B01
UniProtKB Acc Q02KE6
UniProtKB ID Q02KE6_PSEAB
UniRef100 UniRef100_Q02KE6
UniRef50 UniRef50_Q9I369
UniRef90 UniRef90_Q9I369

Product

Feature Type CDS
Coding Frame 1
Product Name
HsiA2
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -3.85
Kyte-Doolittle Hydrophobicity Value -0.230
Molecular Weight (kDa) 58.6
Isoelectric Point (pI) 6.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse acute lung Infection model
Infection model Mouse burn Infection model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 68 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000081 (1226 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43050
Search term: hsiA2
Search term: HsiA2

References

Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis.
Lesic B, Starkey M, He J, Hazan R, Rahme LG
Microbiology (Reading, Engl.) 2009 Sep;155(Pt 9):2845-55
PubMed ID: 19497948
The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells.
Sana TG, Hachani A, Bucior I, Soscia C, Garvis S, Termine E, Engel J, Filloux A, Bleves S
J. Biol. Chem. 2012 Aug;287(32):27095-105
PubMed ID: 22665491
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999