Pseudomonas aeruginosa UCBPP-PA14, PA14_43070 (hcp2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_43070
Name
hcp2
Synonym: hcpD
Replicon chromosome
Genomic location 3831886 - 3832404 (+ strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq YP_791591.1
GI 116049604
Entrez 4381812
NCBI Locus Tag PA14_43070

Product

Feature Type CDS
Coding Frame 1
Product Name
Hcp2
Product Name Confidence: Class 1
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)
Evidence for Translation
Charge (pH 7) -5.88
Kyte-Doolittle Hydrophobicity Value -0.449
Molecular Weight (kDa) 19.1
Isoelectric Point (pI) 5.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3HE1 structural genomics, unknown function 05/07/09 Secreted protein Hcp3 from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.098 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse acute lung Infection model
Infection model Mouse burn Infection model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 67 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000260 (1028 members)
Putative Inparalogs 1 predicted inparalogs
secreted protein Hcp (PA14_69560) 100.0% identity

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43070
Search term: hcp2
Search term: Hcp2

References

Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis.
Lesic B, Starkey M, He J, Hazan R, Rahme LG
Microbiology (Reading, Engl.) 2009 Sep;155(Pt 9):2845-55
PubMed ID: 19497948
The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells.
Sana TG, Hachani A, Bucior I, Soscia C, Garvis S, Termine E, Engel J, Filloux A, Bleves S
J. Biol. Chem. 2012 Aug;287(32):27095-105
PubMed ID: 22665491
An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14.
Jones C, Hachani A, Manoli E, Filloux A
J. Bacteriol. 2014 Feb;196(4):800-10
PubMed ID: 24317402
PubMed ID: 28673999