Pseudomonas aeruginosa UCBPP-PA14, PA14_43080 (vgrG14)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_43080
Name
vgrG14
Replicon chromosome
Genomic location 3832600 - 3834642 (+ strand)
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq YP_791592.1
GI 116049603
Entrez 4381811
NCBI Locus Tag PA14_43080

Product

Feature Type CDS
Coding Frame 1
Product Name
VgrG14
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -1.53
Kyte-Doolittle Hydrophobicity Value -0.493
Molecular Weight (kDa) 75699.4
Isoelectric Point (pI) 6.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 19497948
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse burn Infection model
Infection model Mouse acute lung Infection model
Reference 19497948

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 145 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG014913 (29 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_43080
Search term: vgrG14
Search term: VgrG14

References

Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis.
Lesic B, Starkey M, He J, Hazan R, Rahme LG
Microbiology (Reading, Engl.) 2009 Sep;155(Pt 9):2845-55
PubMed ID: 19497948
The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells.
Sana TG, Hachani A, Bucior I, Soscia C, Garvis S, Termine E, Engel J, Filloux A, Bleves S
J. Biol. Chem. 2012 Aug;287(32):27095-105
PubMed ID: 22665491
An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14.
Jones C, Hachani A, Manoli E, Filloux A
J. Bacteriol. 2014 Feb;196(4):800-10
PubMed ID: 24317402
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999