Pseudomonas aeruginosa UCBPP-PA14, PA14_49780

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_49780
Name
Replicon chromosome
Genomic location 4427833 - 4428240 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_792137.1
GI 116049061
Entrez 4380097
INSDC ABJ10297.1
NCBI Locus Tag PA14_49780
UniParc UPI00000C5263
UniProtKB Acc Q02IU9
UniProtKB ID Q02IU9_PSEAB
UniRef100 UniRef100_Q9I4K6
UniRef50 UniRef50_Q9I4K6
UniRef90 UniRef90_Q9I4K6

Product

Feature Type CDS
Coding Frame 1
Product Name
fosfomycin resistance protein
Synonyms
Evidence for Translation
Charge (pH 7) -0.87
Kyte-Doolittle Hydrophobicity Value -0.219
Molecular Weight (kDa) 15.1
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
FosA An enzyme that confers resistance to fosfomycin in Serratia marcescens by breaking the epoxide ring of the molecule. It depends on the cofactors Manganese (II) and Potassium and uses Glutathione (GSH) as the nucleophilic molecule. In Pseudomonas aeruginosa, FosA catalyzes the conjugation of glutathione to carbon-1 of fosfomycin, rendering it ineffective as an antibacterial drug. ARO:3000149 protein homolog model Perfect 283.1 100.0 3.0.1

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1LQK TRANSFERASE 05/10/02 High Resolution Structure of Fosfomycin Resistance Protein A (FosA) PSEUDOMONAS AERUGINOSA 1.35 X-RAY DIFFRACTION 100.0
1LQO TRANSFERASE 05/11/02 Crystal Strutcure of the Fosfomycin Resistance Protein A (FosA) Containing Bound Thallium Cations PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1NKI TRANSFERASE 01/03/03 CRYSTAL STRUCTURE OF THE FOSFOMYCIN RESISTANCE PROTEIN A (FOSA) CONTAINING BOUND PHOSPHONOFORMATE PSEUDOMONAS AERUGINOSA 0.95 X-RAY DIFFRACTION 100.0
1NNR TRANSFERASE 01/14/03 Crystal structure of a probable fosfomycin resistance protein (PA1129) from Pseudomonas aeruginosa with sulfate present in the active site PSEUDOMONAS AERUGINOSA 2.25 X-RAY DIFFRACTION 100.0
1LQP TRANSFERASE 05/11/02 CRYSTAL STRUCTURE OF THE FOSFOMYCIN RESISTANCE PROTEIN (FOSA) CONTAINING BOUND SUBSTRATE PSEUDOMONAS AERUGINOSA 1.19 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 84 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001089 (355 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_49780

References

No references are associated with this feature.