Pseudomonas aeruginosa PA7, PSPA7_4642 (exlA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PA7
Locus Tag
PSPA7_4642
Name
exlA
Synonym: exolysin
Replicon chromosome
Genomic location 4766323 - 4771278 (+ strand)

Cross-References

RefSeq YP_001349986.1
GI 152989608
Entrez 5354208
INSDC ABR86791.1
NCBI Locus Tag PSPA7_4642
UniParc UPI0000D72521
UniProtKB Acc A6VAA1
UniProtKB ID A6VAA1_PSEA7
UniRef100 UniRef100_A6VAA1
UniRef50 UniRef50_A0A0B7DBC5
UniRef90 UniRef90_A6VAA1

Product

Feature Type CDS
Coding Frame 1
Product Name
ExlA
Product Name Confidence: Class 1
Synonyms hemolysin
Evidence for Translation
Charge (pH 7) 0.85
Kyte-Doolittle Hydrophobicity Value -0.549
Molecular Weight (kDa) 171649.6
Isoelectric Point (pI) 7.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Western blot evidence
Outer Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000011
genetic interaction evidence
Host Organism Mus musculus
Notes Leads to lethal lung hemorrhage in a mouse model of acute pneumonia.
Infection model Mouse model of acute pneumonia
Reference 24528863
Source 2
Database PseudoCAP
Category
Evidence ECO:0000011
genetic interaction evidence
Host Organism Homo sapiens
Notes Induces cell retraction, actin cytoskeleton disorganization, and cell-cell junction disruption in HUVECs.
Infection model LDH cytotoxicity assay in human umbilical vein endothelial cells (HUVEC)
Reference 24528863

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 68 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG022933 (55 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PSPA7_4642
Search term: exlA
Search term: ExlA

References

A type III secretion negative clinical strain of Pseudomonas aeruginosa employs a two-partner secreted exolysin to induce hemorrhagic pneumonia.
Elsen S, Huber P, Bouillot S, Couté Y, Fournier P, Dubois Y, Timsit JF, Maurin M, Attrée I.
Cell Host Microbe. 2014 Feb 12;15(2):164-76.
PubMed ID: 24528863