Pseudomonas putida KT2440, PP_2679 (pedH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas putida KT2440 (TIGR)
GCF_000007565.2|latest
Locus Tag
PP_2679
Name
pedH
Synonym: qedH-II
Replicon chromosome
Genomic location 3066962 - 3068749 (+ strand)
CommentProtein is involved in the lanthanide-dependent signaling leading to transcriptional autoactivation
CommentNew function/Role: Initiates an alternative route for glycerol metabolism that provides a growth advantage by reducing the lag phase of the cells
CommentPedH-dependent growth with 2-phenylethanol under low (nM) concentrations of lanthanides relies on the ABC transporter PedA1A2BC

Cross-References

RefSeq NP_744823.1
GI 26989398

Product

Feature Type CDS
Coding Frame 1
Product Name
PedH
Product Name Confidence: Class 1
Synonyms quinoprotein ethanol dehydrogenase
Evidence for Translation
Charge (pH 7)
Kyte-Doolittle Hydrophobicity Value -0.293
Molecular Weight (kDa) 64.9
Isoelectric Point (pI) 8.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6ZCW OXIDOREDUCTASE 06/12/20 Crystal structure of lanthanide-dependent alcohol dehydrogenase PedH from Pseudomonas putida KT2440 Pseudomonas putida KT2440 1.65 X-RAY DIFFRACTION 99.8
6ZCV OXIDOREDUCTASE 06/12/20 Crystal structure of lanthanide-dependent alcohol dehydrogenase PedH from Pseudomonas putida KT2440 Pseudomonas putida KT2440 1.7 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PP_2679
Search term: pedH
Search term: PedH

Human Homologs

References

Functional Role of Lanthanides in Enzymatic Activity and Transcriptional Regulation of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenases in Pseudomonas putida KT2440.
Wehrmann M, Billard P, Martin-Meriadec A, Zegeye A, Klebensberger J
mBio 2017 Jun 27;8(3)
PubMed ID: 28655819
The Cellular Response to Lanthanum Is Substrate Specific and Reveals a Novel Route for Glycerol Metabolism in Pseudomonas putida KT2440.
Wehrmann M, Toussaint M, Pfannstiel J, Billard P, Klebensberger J
mBio 2020 Apr 28;11(2)
PubMed ID: 32345644
Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability.
Wehrmann M, Berthelot C, Billard P, Klebensberger J
Front Microbiol 2019;10:2494
PubMed ID: 31736923