Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0652
|
Name |
vfr
|
Replicon | chromosome |
Genomic location | 706028 - 706672 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249343.1 |
GI | 15595849 |
Affymetrix | PA0652_vfr_at |
DNASU | PaCD00006293 |
Entrez | 880744 |
GenBank | AAG04041.1 |
INSDC | AAG04041.1 |
NCBI Locus Tag | PA0652 |
protein_id(GenBank) | gb|AAG04041.1|AE004500_7|gnl|PseudoCAP|PA0652 |
TIGR | NTL03PA00653 |
UniParc | UPI0000138482 |
UniProtKB Acc | P55222 |
UniProtKB ID | VFR_PSEAE |
UniRef100 | UniRef100_P55222 |
UniRef50 | UniRef50_P29281 |
UniRef90 | UniRef90_P55222 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
transcriptional regulator Vfr
|
Synonyms |
cyclic AMP receptor-like protein |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 3.29 |
Kyte-Doolittle Hydrophobicity Value | -0.250 |
Molecular Weight (kDa) | 24.2 |
Isoelectric Point (pI) | 8.22 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7FF9 | DNA BINDING PROTEIN | 07/23/21 | Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I) | Pseudomonas aeruginosa PAO1 | 2.4 | X-RAY DIFFRACTION | 100.0 |
2OZ6 | DNA BINDING PROTEIN | 02/24/07 | Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP | Pseudomonas aeruginosa | 2.8 | X-RAY DIFFRACTION | 100.0 |
7FF0 | DNA BINDING PROTEIN | 07/22/21 | Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin | Pseudomonas aeruginosa PAO1 | 2.6 | X-RAY DIFFRACTION | 100.0 |
7FF8 | DNA BINDING PROTEIN | 07/22/21 | Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I) | Pseudomonas aeruginosa PAO1 | 2.8 | X-RAY DIFFRACTION | 99.5 |
7FEW | DNA BINDING PROTEIN | 07/22/21 | Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin gold analogues | Pseudomonas aeruginosa PAO1 | 1.75 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | PseudoCAP |
Category | Not available / Not known |
Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
Host Organism | Mus musculus |
Notes | |
Infection model | Mouse model of acute pneumonia |
Reference | 14977975 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 443 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000272 (963 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0652
Search term: vfr
Search term: transcriptional regulator Vfr
|
Vfr controls quorum sensing in Pseudomonas aeruginosa.
Albus AM, Pesci EC, Runyen-Janecky LJ, West SE, Iglewski BH
J Bacteriol 1997 Jun;179(12):3928-35
PubMed ID: 9190808
|
The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family.
West SE, Sample AK, Runyen-Janecky LJ
J Bacteriol 1994 Dec;176(24):7532-42
PubMed ID: 8002577
|