Pseudomonas aeruginosa PAO1, PA0773 (pdxJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0773
Name
pdxJ
Replicon chromosome
Genomic location 841079 - 841825 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249464.1
GI 15595970
Affymetrix PA0773_pdxJ_at
Entrez 878131
GenBank AAG04162.1
INSDC AAG04162.1
NCBI Locus Tag PA0773
protein_id(GenBank) gb|AAG04162.1|AE004512_5|gnl|PseudoCAP|PA0773
TIGR NTL03PA00774
UniParc UPI0000131516
UniProtKB Acc Q9I5G5
UniProtKB ID PDXJ_PSEAE
UniRef100 UniRef100_Q02HS5
UniRef50 UniRef50_A9KFA7
UniRef90 UniRef90_Q02HS5

Product

Feature Type CDS
Coding Frame 1
Product Name
pyridoxal phosphate biosynthetic protein PdxJ
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.61
Kyte-Doolittle Hydrophobicity Value -0.237
Molecular Weight (kDa) 27.2
Isoelectric Point (pI) 5.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5DLC TRANSFERASE 09/04/15 X-ray Crystal Structure of a Pyridoxine 5-prime-phosphate synthase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 355 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000747 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0773
Search term: pdxJ

Human Homologs

References

Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
Takiff HE, Baker T, Copeland T, Chen SM, Court DL
J Bacteriol 1992 Mar;174(5):1544-53
PubMed ID: 1537799
Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.
Lam HM, Tancula E, Dempsey WB, Winkler ME
J Bacteriol 1992 Mar;174(5):1554-67
PubMed ID: 1537800