Pseudomonas aeruginosa PAO1, PA0870 (phhC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0870
Name
phhC
Synonym: aspC
Synonym: tyrB
Replicon chromosome
Genomic location 950962 - 952161 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249561.1
GI 15596067
Affymetrix PA0870_phhC_at
DNASU PaCD00006400
Entrez 880839
GenBank AAG04259.1
INSDC AAG04259.1
NCBI Locus Tag PA0870
protein_id(GenBank) gb|AAG04259.1|AE004522_1|gnl|PseudoCAP|PA0870
TIGR NTL03PA00871
UniParc UPI000013195D
UniProtKB Acc P43336
UniProtKB ID PHHC_PSEAE
UniRef100 UniRef100_P43336
UniRef50 UniRef50_P43336
UniRef90 UniRef90_P43336

Product

Feature Type CDS
Coding Frame 1
Product Name
aromatic amino acid aminotransferase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.28
Kyte-Doolittle Hydrophobicity Value -0.018
Molecular Weight (kDa) 43.3
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 174 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000837 (453 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0870
Search term: phhC

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutamic-oxaloacetic transaminase 1 like 1 [Source:HGNC Symbol;Acc:HGNC:28487]
E-value: 1.1e-33
Percent Identity: 26.7

References

Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster.
Zhao G, Xia T, Song J, Jensen RA
Proc Natl Acad Sci U S A 1994 Feb 15;91(4):1366-70
PubMed ID: 8108417
PhhC is an essential aminotransferase for aromatic amino acid catabolism in Pseudomonas aeruginosa.
Gu W, Song J, Bonner CA, Xie G, Jensen RA
Microbiology (Reading) 1998 Nov;144 ( Pt 11):3127-3134
PubMed ID: 9846749
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994