Pseudomonas aeruginosa PAO1, PA0903 (alaS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0903
Name
alaS
Synonym: sya
Replicon chromosome
Genomic location 986818 - 989442 (+ strand)

Cross-References

RefSeq NP_249594.1
GI 15596100
Affymetrix PA0903_alaS_at
Entrez 878299
GenBank AAG04292.1
INSDC AAC46017.1
NCBI Locus Tag PA0903
protein_id(GenBank) gb|AAG04292.1|AE004524_9|gnl|PseudoCAP|PA0903
TIGR NTL03PA00904
UniParc UPI0000136317
UniProtKB Acc Q9I553
UniProtKB ID SYA_PSEAE
UniRef100 UniRef100_Q9I553
UniRef50 UniRef50_A7ZQC5
UniRef90 UniRef90_Q9I553

Product

Feature Type CDS
Coding Frame 1
Product Name
alanyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -24.87
Kyte-Doolittle Hydrophobicity Value -0.256
Molecular Weight (kDa) 94.7
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000870 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0903
Search term: alaS

Human Homologs

Ensembl 110, assembly GRCh38.p14
alanyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:20]
E-value: 1.0e-132
Percent Identity: 39.1

References

Evidence for a "cysteine-histidine box" metal-binding site in an Escherichia coli aminoacyl-tRNA synthetase.
Miller WT, Hill KA, Schimmel P
Biochemistry 1991 Jul 16;30(28):6970-6
PubMed ID: 1712632
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994