Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0934
|
Name |
relA
|
Replicon | chromosome |
Genomic location | 1023053 - 1025296 (+ strand) |
Transposon Mutants | 5 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
RefSeq | NP_249625.1 |
GI | 15596131 |
Affymetrix | PA0934_relA_at |
Entrez | 881299 |
GenBank | AAG04323.1 |
INSDC | AAG04323.1 |
NCBI Locus Tag | PA0934 |
protein_id(GenBank) | gb|AAG04323.1|AE004528_1|gnl|PseudoCAP|PA0934 |
TIGR | NTL03PA00935 |
UniParc | UPI00000C51C6 |
UniProtKB Acc | Q9I524 |
UniProtKB ID | Q9I524_PSEAE |
UniRef100 | UniRef100_Q9I524 |
UniRef50 | UniRef50_D7I2K8 |
UniRef90 | UniRef90_A0A024HCY6 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
GTP pyrophosphokinase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 4.82 |
Kyte-Doolittle Hydrophobicity Value | -0.312 |
Molecular Weight (kDa) | 83.8 |
Isoelectric Point (pI) | 7.57 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | Victors : 4255 |
Category | Stringent Response |
Evidence |
ECO:0000015
mutant phenotype evidence
|
Host Organism | Drosophila melanogaster |
Notes | relA mutant attenuated for virulence in D. melanogaster feeding assay |
Infection model | Drosophila melanogaster feeding assay |
Reference | 15385461 |
Source 2 | |
Database | PseudoCAP |
Category | Stringent Response |
Evidence |
ECO:0000316
genetic interaction evidence used in manual assertion
|
Host Organism | Rattus norvegicus |
Notes | spoT relA double mutant was significantly less virulent than both wild-type strain PAO1 and the relA mutant in D. melanogaster feeding assay. |
Infection model | Drosophila melanogaster feeding assay |
Reference | 21788391 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 565 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000900 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0934
Search term: relA
Search term: GTP pyrophosphokinase
|
The nucleotide sequence and characterization of the relA gene of Escherichia coli.
Metzger S, Dror IB, Aizenman E, Schreiber G, Toone M, Friesen JD, Cashel M, Glaser G
J Biol Chem 1988 Oct 25;263(30):15699-704
PubMed ID: 2844820
|
Pseudomonas aeruginosa relA contributes to virulence in Drosophila melanogaster.
Erickson DL, Lines JL, Pesci EC, Venturi V, Storey DG
Infect Immun 2004 Oct;72(10):5638-45
PubMed ID: 15385461
|
The stringent response is essential for Pseudomonas aeruginosa virulence in the rat lung agar bead and Drosophila melanogaster feeding models of infection.
Vogt SL, Green C, Stevens KM, Day B, Erickson DL, Woods DE, Storey DG
Infect Immun 2011 Oct;79(10):4094-104
PubMed ID: 21788391
|