Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1000
|
Name |
pqsE
|
Replicon | chromosome |
Genomic location | 1082949 - 1083854 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | required for phenazine, and cyanide production |
Comment | Quinolone signal response |
Comment | not required for Pseudomonas quinolone signal(PQS) synthesis |
Comment | may have a role in the cellular response to PQS |
RefSeq | NP_249691.1 |
GI | 15596197 |
Affymetrix | PA1000_at |
Entrez | 880721 |
GenBank | AAG04389.1 |
INSDC | AAG04389.1 |
NCBI Locus Tag | PA1000 |
protein_id(GenBank) | gb|AAG04389.1|AE004532_13|gnl|PseudoCAP|PA1000 |
TIGR | NTL03PA01001 |
UniParc | UPI0000139EE3 |
UniProtKB Acc | P20581 |
UniProtKB ID | Y1000_PSEAE |
UniRef100 | UniRef100_P20581 |
UniRef50 | UniRef50_P20581 |
UniRef90 | UniRef90_P20581 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Quinolone signal response protein
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -10.61 |
Kyte-Doolittle Hydrophobicity Value | -0.178 |
Molecular Weight (kDa) | 34.3 |
Isoelectric Point (pI) | 5.25 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2Q0I | METAL BINDING PROTEIN | 05/22/07 | Structure of Pseudomonas Quinolone Signal Response Protein PqsE | Pseudomonas aeruginosa | 1.57 | X-RAY DIFFRACTION | 100.0 |
8DQ1 | TRANSCRIPTION/DNA | 07/18/22 | Quorum-sensing receptor RhlR bound to PqsE | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 4.1 | ELECTRON MICROSCOPY | 100.0 |
8DQ0 | TRANSCRIPTION | 07/18/22 | Quorum-sensing receptor RhlR bound to PqsE | Pseudomonas aeruginosa | 3.74 | ELECTRON MICROSCOPY | 100.0 |
7TZA | HYDROLASE/INHIBITOR | 02/15/22 | Structure of PQS Response Protein PqsE in complex with N-(4-(3-neopentylureido)phenyl)-1H-indazole-7-carboxamide | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 100.0 |
7R3F | HYDROLASE | 02/07/22 | Monomeric PqsE mutant E187R | Pseudomonas aeruginosa PAO1 | 1.65 | X-RAY DIFFRACTION | 99.7 |
5HIP | METAL BINDING PROTEIN | 01/12/16 | Crystal Structure of PQS Response Protein PqsE in Complex with 2-(pyridin-3-yl)benzoic acid | Pseudomonas aeruginosa PAO1 | 1.992 | X-RAY DIFFRACTION | 100.0 |
7R3E | TRANSCRIPTION | 02/07/22 | Fusion construct of PqsE and RhlR in complex with the synthetic antagonist mBTL | Pseudomonas aeruginosa PAO1 | 3.46 | X-RAY DIFFRACTION | 100.0 |
7KGX | HYDROLASE/Inhibitor | 10/19/20 | Structure of PQS Response Protein PqsE in Complex with 4-(3-(2-methyl-2-morpholinobutyl)ureido)-N-(thiazol-2-yl)benzamide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2 | X-RAY DIFFRACTION | 100.0 |
7KGW | HYDROLASE/Inhibitor | 10/19/20 | Structure of PQS Response Protein PqsE in Complex N-(3-(1H-pyrazol-5-yl)phenyl)-1H-indazole-7-carboxamide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.99 | X-RAY DIFFRACTION | 100.0 |
7TZ9 | HYDROLASE | 02/15/22 | Structure of PQS Response Protein PqsE(E182W) Variant | Pseudomonas aeruginosa | 2.01 | X-RAY DIFFRACTION | 99.7 |
7U6G | HYDROLASE | 03/04/22 | Structure of PQS Response Protein PqsE(E182W,E280A) Variant | Pseudomonas aeruginosa | 2.45 | X-RAY DIFFRACTION | 99.3 |
2VW8 | SIGNALING PROTEIN | 06/17/08 | Crystal Structure of Quinolone signal response protein pqsE from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA PAO1 | 1.45 | X-RAY DIFFRACTION | 100.0 |
8B4A | SIGNALING PROTEIN | 09/20/22 | Nativ complex of PqsE and RhlR with autoinducer C4-HSL | Pseudomonas aeruginosa PAO1 | 3.06 | X-RAY DIFFRACTION | 100.0 |
7R3J | TRANSCRIPTION | 02/07/22 | Nativ complex of PqsE and RhlR with the synthetic antagonist mBTL | Pseudomonas aeruginosa PAO1 | 3.06 | X-RAY DIFFRACTION | 100.0 |
5HIS | METAL BINDING PROTEIN | 01/12/16 | Crystal Structure of PQS Response Protein PqsE in Complex with 3-methylthiophene-2-carboxylic acid | Pseudomonas aeruginosa PAO1 | 1.77 | X-RAY DIFFRACTION | 100.0 |
5HIQ | METAL BINDING PROTEIN | 01/12/16 | Crystal Structure of PQS Response Protein PqsE in Complex with 2-(1H-pyrrol-1-yl)benzoic acid | Pseudomonas aeruginosa PAO1 | 2.1 | X-RAY DIFFRACTION | 100.0 |
3DH8 | METAL BINDING PROTEIN | 06/17/08 | Structure of Pseudomonas Quinolone Signal Response Protein PqsE | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 99.7 |
2Q0J | METAL BINDING PROTEIN | 05/22/07 | Structure of Pseudomonas Quinolone Signal Response Protein PqsE | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 100.0 |
5HIO | METAL BINDING PROTEIN | 01/12/16 | Crystal Structure of PQS Response Protein PqsE in Complex with 2-aminobenzoylacetate | Pseudomonas aeruginosa PAO1 | 1.9 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
bis(4-nitrophenyl) hydrogen phosphate | DB07418 | DrugBank | 5.1.4 | P20581 | Uncharacterized protein PA1000 | 100.0 | 301 | 3.7e-178 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 100 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000963 (297 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1000
Search term: pqsE
Search term: Quinolone signal response protein
|
Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP
J Bacteriol 1990 Feb;172(2):884-900
PubMed ID: 2153661
|
PqsE Expands and Differentially Modulates the RhlR Quorum Sensing Regulon in Pseudomonas aeruginosa.
Letizia M, Mellini M, Fortuna A, Visca P, Imperi F, Leoni L, Rampioni G
Microbiol Spectr 2022 Jun 29;10(3):e0096122
PubMed ID: 35604161
|