Pseudomonas aeruginosa PAO1, PA1000 (pqsE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1000
Name
pqsE
Replicon chromosome
Genomic location 1082949 - 1083854 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Commentrequired for phenazine, and cyanide production
CommentQuinolone signal response
Commentnot required for Pseudomonas quinolone signal(PQS) synthesis
Commentmay have a role in the cellular response to PQS

Cross-References

RefSeq NP_249691.1
GI 15596197
Affymetrix PA1000_at
Entrez 880721
GenBank AAG04389.1
INSDC AAG04389.1
NCBI Locus Tag PA1000
protein_id(GenBank) gb|AAG04389.1|AE004532_13|gnl|PseudoCAP|PA1000
TIGR NTL03PA01001
UniParc UPI0000139EE3
UniProtKB Acc P20581
UniProtKB ID Y1000_PSEAE
UniRef100 UniRef100_P20581
UniRef50 UniRef50_P20581
UniRef90 UniRef90_P20581

Product

Feature Type CDS
Coding Frame 1
Product Name
Quinolone signal response protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.61
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 34.3
Isoelectric Point (pI) 5.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2Q0I METAL BINDING PROTEIN 05/22/07 Structure of Pseudomonas Quinolone Signal Response Protein PqsE Pseudomonas aeruginosa 1.57 X-RAY DIFFRACTION 100.0
8DQ1 TRANSCRIPTION/DNA 07/18/22 Quorum-sensing receptor RhlR bound to PqsE Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 4.1 ELECTRON MICROSCOPY 100.0
8DQ0 TRANSCRIPTION 07/18/22 Quorum-sensing receptor RhlR bound to PqsE Pseudomonas aeruginosa 3.74 ELECTRON MICROSCOPY 100.0
7TZA HYDROLASE/INHIBITOR 02/15/22 Structure of PQS Response Protein PqsE in complex with N-(4-(3-neopentylureido)phenyl)-1H-indazole-7-carboxamide Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
7R3F HYDROLASE 02/07/22 Monomeric PqsE mutant E187R Pseudomonas aeruginosa PAO1 1.65 X-RAY DIFFRACTION 99.7
5HIP METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-(pyridin-3-yl)benzoic acid Pseudomonas aeruginosa PAO1 1.992 X-RAY DIFFRACTION 100.0
7R3E TRANSCRIPTION 02/07/22 Fusion construct of PqsE and RhlR in complex with the synthetic antagonist mBTL Pseudomonas aeruginosa PAO1 3.46 X-RAY DIFFRACTION 100.0
7KGX HYDROLASE/Inhibitor 10/19/20 Structure of PQS Response Protein PqsE in Complex with 4-(3-(2-methyl-2-morpholinobutyl)ureido)-N-(thiazol-2-yl)benzamide Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2 X-RAY DIFFRACTION 100.0
7KGW HYDROLASE/Inhibitor 10/19/20 Structure of PQS Response Protein PqsE in Complex N-(3-(1H-pyrazol-5-yl)phenyl)-1H-indazole-7-carboxamide Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.99 X-RAY DIFFRACTION 100.0
7TZ9 HYDROLASE 02/15/22 Structure of PQS Response Protein PqsE(E182W) Variant Pseudomonas aeruginosa 2.01 X-RAY DIFFRACTION 99.7
7U6G HYDROLASE 03/04/22 Structure of PQS Response Protein PqsE(E182W,E280A) Variant Pseudomonas aeruginosa 2.45 X-RAY DIFFRACTION 99.3
2VW8 SIGNALING PROTEIN 06/17/08 Crystal Structure of Quinolone signal response protein pqsE from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 1.45 X-RAY DIFFRACTION 100.0
8B4A SIGNALING PROTEIN 09/20/22 Nativ complex of PqsE and RhlR with autoinducer C4-HSL Pseudomonas aeruginosa PAO1 3.06 X-RAY DIFFRACTION 100.0
7R3J TRANSCRIPTION 02/07/22 Nativ complex of PqsE and RhlR with the synthetic antagonist mBTL Pseudomonas aeruginosa PAO1 3.06 X-RAY DIFFRACTION 100.0
5HIS METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 3-methylthiophene-2-carboxylic acid Pseudomonas aeruginosa PAO1 1.77 X-RAY DIFFRACTION 100.0
5HIQ METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-(1H-pyrrol-1-yl)benzoic acid Pseudomonas aeruginosa PAO1 2.1 X-RAY DIFFRACTION 100.0
3DH8 METAL BINDING PROTEIN 06/17/08 Structure of Pseudomonas Quinolone Signal Response Protein PqsE Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 99.7
2Q0J METAL BINDING PROTEIN 05/22/07 Structure of Pseudomonas Quinolone Signal Response Protein PqsE Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
5HIO METAL BINDING PROTEIN 01/12/16 Crystal Structure of PQS Response Protein PqsE in Complex with 2-aminobenzoylacetate Pseudomonas aeruginosa PAO1 1.9 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
bis(4-nitrophenyl) hydrogen phosphate DB07418 DrugBank 5.1.4 P20581 Uncharacterized protein PA1000 100.0 301 3.7e-178

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 100 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000963 (297 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1000
Search term: pqsE

Human Homologs

References

Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP
J Bacteriol 1990 Feb;172(2):884-900
PubMed ID: 2153661
PqsE Expands and Differentially Modulates the RhlR Quorum Sensing Regulon in Pseudomonas aeruginosa.
Letizia M, Mellini M, Fortuna A, Visca P, Imperi F, Leoni L, Rampioni G
Microbiol Spectr 2022 Jun 29;10(3):e0096122
PubMed ID: 35604161