Pseudomonas aeruginosa PAO1, PA1092 (fliC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1092
Name
fliC
Replicon chromosome
Genomic location 1183058 - 1184524 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_249783.1
GI 15596289
Affymetrix PA1092_fliC_at
Entrez 882052
GenBank AAG04481.1
INSDC AAG04481.1
NCBI Locus Tag PA1092
protein_id(GenBank) gb|AAG04481.1|AE004540_1|gnl|PseudoCAP|PA1092
TIGR NTL03PA01093
UniParc UPI000012A88C
UniProtKB Acc P72151
UniProtKB ID FLICB_PSEAE
UniRef100 UniRef100_P72151
UniRef50 UniRef50_P72151
UniRef90 UniRef90_P72151

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellin type B
Product Name Confidence: Class 1
Synonyms flagellar filament protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (49.24 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.00
Kyte-Doolittle Hydrophobicity Value -0.077
Molecular Weight (kDa) 49.2
Isoelectric Point (pI) 5.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Periplasmic Class 1
Outer Membrane Vesicle Class 1
Flagellar
Note: This is based on PSORTb V3.0 secondary localization prediction
 Class 3
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by lysozyme and MgCl2.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WK6 PROTEIN FIBRIL 07/24/17 Cryo-EM structure of P. aeruginosa flagellar filaments G420A Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 4.3 ELECTRON MICROSCOPY 99.8

Virulence Evidence

Source 1
Database Victors : 4261
Category Flagella
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Infection model Neonatal mouse model of pneumonia.
Reference 9423837
Source 2
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 293 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001053 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1092
Search term: fliC
Search term: flagellin type B

Human Homologs

References

Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection.
Feldman M, Bryan R, Rajan S, Scheffler L, Brunnert S, Tang H, Prince A
Infect Immun 1998 Jan;66(1):43-51
PubMed ID: 9423837
Cloning and comparison of fliC genes and identification of glycosylation in the flagellin of Pseudomonas aeruginosa a-type strains.
Brimer CD, Montie TC
J Bacteriol 1998 Jun;180(12):3209-17
PubMed ID: 9620973
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878