Pseudomonas aeruginosa PAO1, PA1155 (nrdB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1155
Name
nrdB
Replicon chromosome
Genomic location 1249907 - 1251154 (- strand)

Cross-References

RefSeq NP_249846.1
GI 15596352
Affymetrix PA1155_nrdB_at
Entrez 877692
GenBank AAG04544.1
INSDC AAG04544.1
NCBI Locus Tag PA1155
protein_id(GenBank) gb|AAG04544.1|AE004545_7|gnl|PseudoCAP|PA1155
TIGR NTL03PA01156
UniParc UPI00000C5279
UniProtKB Acc Q9I4I2
UniProtKB ID Q9I4I2_PSEAE
UniRef100 UniRef100_Q9I4I2
UniRef50 UniRef50_Q9I4I2
UniRef90 UniRef90_Q9I4I2

Product

Feature Type CDS
Coding Frame 1
Product Name
NrdB, tyrosyl radical-harboring component of class Ia ribonucleotide reductase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -21.44
Kyte-Doolittle Hydrophobicity Value -0.431
Molecular Weight (kDa) 47.4
Isoelectric Point (pI) 4.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001114 (560 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1155
Search term: nrdB

Human Homologs

Ensembl 86, assembly GRCh38.p7
ribonucleotide reductase regulatory subunit M2 [Source:HGNC Symbol;Acc:HGNC:10452]
E-value: 9.0e-34
Percent Identity: 28.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Ribonucleotide reductase modularity: Atypical duplication of the ATP-cone domain in Pseudomonas aeruginosa.
Torrents E, Westman M, Sahlin M, Sjöberg BM
J. Biol. Chem. 2006 Sep;281(35):25287-96
PubMed ID: 16829681
Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability.
Elledge SJ, Davis RW
Mol. Cell. Biol. 1987 Aug;7(8):2783-93
PubMed ID: 3313004
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994