Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1250
|
Name |
aprI
|
Replicon | chromosome |
Genomic location | 1357317 - 1357712 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_249941.1 |
GI | 15596447 |
Affymetrix | PA1250_aprI_at |
DNASU | PaCD00006577 |
Entrez | 881261 |
GenBank | AAG04639.1 |
INSDC | AAG04639.1 |
NCBI Locus Tag | PA1250 |
protein_id(GenBank) | gb|AAG04639.1|AE004554_7|gnl|PseudoCAP|PA1250 |
TIGR | NTL03PA01251 |
UniParc | UPI000012D678 |
UniProtKB Acc | Q03026 |
UniProtKB ID | INH_PSEAE |
UniRef100 | UniRef100_Q03026 |
UniRef50 | UniRef50_Q03026 |
UniRef90 | UniRef90_Q03026 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
alkaline proteinase inhibitor AprI
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -1.89 |
Kyte-Doolittle Hydrophobicity Value | 0.012 |
Molecular Weight (kDa) | 14.0 |
Isoelectric Point (pI) | 5.12 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1JIW | HYDROLASE/HYDROLASE INHIBITOR | 07/03/01 | Crystal structure of the APR-APRin complex | Pseudomonas aeruginosa | 1.74 | X-RAY DIFFRACTION | 100.0 |
2RN4 | HYDROLASE INHIBITOR | 12/06/07 | Solution structure of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa | Pseudomonas aeruginosa | NOT | SOLUTION NMR | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001202 (325 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1250
Search term: aprI
Search term: alkaline proteinase inhibitor AprI
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Characterization of a protein inhibitor of extracellular proteases produced by Erwinia chrysanthemi.
Létoffé S, Delepelaire P, Wandersman C
Mol Microbiol 1989 Jan;3(1):79-86
PubMed ID: 2654540
|
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov 2;121(1):47-54
PubMed ID: 1427098
|