Pseudomonas aeruginosa PAO1, PA1310 (phnW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1310
Name
phnW
Replicon chromosome
Genomic location 1420326 - 1421441 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250001.1
GI 15596507
Affymetrix PA1310_phnW_at
Entrez 881491
GenBank AAG04699.1
INSDC AAG04699.1
NCBI Locus Tag PA1310
protein_id(GenBank) gb|AAG04699.1|AE004560_7|gnl|PseudoCAP|PA1310
TIGR NTL03PA01311
UniParc UPI00000C5305
UniProtKB Acc Q9I434
UniProtKB ID PHNW_PSEAE
UniRef100 UniRef100_Q9I434
UniRef50 UniRef50_Q9I434
UniRef90 UniRef90_Q9I434

Product

Feature Type CDS
Coding Frame 1
Product Name
2-aminoethylphosphonate:pyruvate aminotransferase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.83
Kyte-Doolittle Hydrophobicity Value 0.014
Molecular Weight (kDa) 40.4
Isoelectric Point (pI) 6.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7E7G TRANSFERASE 02/26/21 2-aminoethylphosphonate:pyruvate aminotransferase (AEPT) native Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.35 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 425 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001253 (446 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1310
Search term: phnW

Human Homologs

References

Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa.
Dumora C, Lacoste AM, Cassaigne A
Eur J Biochem 1983 Jun 1;133(1):119-25
PubMed ID: 6406228
Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2.
Jiang W, Metcalf WW, Lee KS, Wanner BL
J Bacteriol 1995 Nov;177(22):6411-21
PubMed ID: 7592415