Pseudomonas aeruginosa PAO1, PA1481 (ccmG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1481
Name
ccmG
Synonym: dsbE
Replicon chromosome
Genomic location 1607065 - 1607607 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250172.1
GI 15596678
Affymetrix PA1481_ccmG_at
DNASU PaCD00006683
Entrez 881065
GenBank AAG04870.1
INSDC AAG04870.1
NCBI Locus Tag PA1481
protein_id(GenBank) gb|AAG04870.1|AE004577_5|gnl|PseudoCAP|PA1481
TIGR NTL03PA01482
UniParc UPI00001298E6
UniProtKB Acc Q9I3N1
UniProtKB ID DSBE_PSEAE
UniRef100 UniRef100_Q9I3N1
UniRef50 UniRef50_Q9I3N1
UniRef90 UniRef90_Q9I3N1

Product

Feature Type CDS
Coding Frame 1
Product Name
cytochrome C biogenesis protein CcmG
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.35
Kyte-Doolittle Hydrophobicity Value 0.069
Molecular Weight (kDa) 20.1
Isoelectric Point (pI) 5.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3KH7 OXIDOREDUCTASE 10/30/09 Crystal structure of the periplasmic soluble domain of reduced CcmG from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.75 X-RAY DIFFRACTION 100.0
3KH9 OXIDOREDUCTASE 10/30/09 Crystal structure of the periplasmic soluble domain of oxidized CcmG from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 406 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001396 (560 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1481
Search term: ccmG

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 42492
Genomic Position 1607311
Position in ORF 247(543)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Paracoccus denitrificans CcmG is a periplasmic protein-disulphide oxidoreductase required for c- and aa3-type cytochrome biogenesis; evidence for a reductase role in vivo.
Page MD, Ferguson SJ
Mol. Microbiol. 1997 Jun;24(5):977-90
PubMed ID: 9220005
The active-site cysteines of the periplasmic thioredoxin-like protein CcmG of Escherichia coli are important but not essential for cytochrome c maturation in vivo.
Fabianek RA, Hennecke H, Thöny-Meyer L
J. Bacteriol. 1998 Apr;180(7):1947-50
PubMed ID: 9537397