Pseudomonas aeruginosa PAO1, PA1549 (copA2)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1549
Name	copA2 Synonym: fixI
Replicon	chromosome
Genomic location	1684941 - 1687376 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_250240.1
GI	15596746
Affymetrix	PA1549_at
Entrez	883015
GenBank	AAG04938.1
INSDC	AAG04938.1
NCBI Locus Tag	PA1549
protein_id(GenBank)	gb|AAG04938.1|AE004583_3|gnl|PseudoCAP|PA1549
TIGR	NTL03PA01550
UniParc	UPI00000C53D3
UniProtKB Acc	Q9I3G8
UniProtKB ID	Q9I3G8_PSEAE
UniRef100	UniRef100_Q9I3G8
UniRef50	UniRef50_Q48GH9
UniRef90	UniRef90_A6V7V2

Product

Feature Type	CDS
Coding Frame	1
Product Name	CopA2 Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-4.29
Kyte-Doolittle Hydrophobicity Value	0.161
Molecular Weight (kDa)	87.2
Isoelectric Point (pI)	6.56

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 643 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003811 (526 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1549 Search term: copA2 Search term: CopA2

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000393343 ATPase copper transporting beta [Source:HGNC Symbol;Acc:HGNC:870] E-value: 3.2e-56 Percent Identity: 25.3

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation. Kahn D, David M, Domergue O, Daveran ML, Ghai J, Hirsch PR, Batut J J Bacteriol 1989 Feb;171(2):929-39 PubMed ID: 2536685

Isolation of a candidate gene for Menkes disease that encodes a potential heavy metal binding protein. Chelly J, Tümer Z, Tønnesen T, Petterson A, Ishikawa-Brush Y, Tommerup N, Horn N, Monaco AP Nat Genet 1993 Jan;3(1):14-9 PubMed ID: 8490646

Solution structure of the fourth metal-binding domain from the Menkes copper-transporting ATPase. Gitschier J, Moffat B, Reilly D, Wood WI, Fairbrother WJ Nat Struct Biol 1998 Jan;5(1):47-54 PubMed ID: 9437429

Distinct functional roles of homologous Cu+ efflux ATPases in Pseudomonas aeruginosa. González-Guerrero M, Raimunda D, Cheng X, Argüello JM Mol Microbiol 2010 Dec;78(5):1246-58 PubMed ID: 21091508