Pseudomonas aeruginosa PAO1, PA1562 (acnA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1562
Name
acnA
Synonym: acn
Replicon chromosome
Genomic location 1700320 - 1703052 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_250253.1
GI 15596759
Affymetrix PA1562_acnA_at
Entrez 879412
GenBank AAG04951.1
INSDC AAG04951.1
NCBI Locus Tag PA1562
protein_id(GenBank) gb|AAG04951.1|AE004584_7|gnl|PseudoCAP|PA1562
TIGR NTL03PA01563
UniParc UPI00000C53E0
UniProtKB Acc Q9I3F5
UniProtKB ID ACNA_PSEAE
UniRef100 UniRef100_Q9I3F5
UniRef50 UniRef50_P25516
UniRef90 UniRef90_Q9I3F5

Product

Feature Type CDS
Coding Frame 1
Product Name
aconitate hydratase 1
Product Name Confidence: Class 2
Synonyms aconitase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -18.55
Kyte-Doolittle Hydrophobicity Value -0.218
Molecular Weight (kDa) 99.1
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 616 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000767 (1110 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1562
Search term: acnA

Human Homologs

Ensembl 86, assembly GRCh38.p7
aconitase 1 [Source:HGNC Symbol;Acc:HGNC:117]
E-value: 1.6e-261
Percent Identity: 53.1

References

The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn).
Prodromou C, Haynes MJ, Guest JR
J. Gen. Microbiol. 1991 Nov;137(11):2505-15
PubMed ID: 1838390
The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases.
Prodromou C, Artymiuk PJ, Guest JR
Eur. J. Biochem. 1992 Mar;204(2):599-609
PubMed ID: 1541275