Pseudomonas aeruginosa PAO1, PA1584 (sdhB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1584
Name
sdhB
Replicon chromosome
Genomic location 1723280 - 1723987 (+ strand)

Cross-References

RefSeq NP_250275.1
GI 15596781
Affymetrix PA1584_sdhB_at
DNASU PaCD00006725
Entrez 882034
GenBank AAG04973.1
INSDC AAG04973.1
NCBI Locus Tag PA1584
protein_id(GenBank) gb|AAG04973.1|AE004586_11|gnl|PseudoCAP|PA1584
TIGR NTL03PA01585
UniParc UPI00000C53F3
UniProtKB Acc Q9I3D4
UniProtKB ID Q9I3D4_PSEAE
UniRef100 UniRef100_W0Z6D2
UniRef50 UniRef50_P51053
UniRef90 UniRef90_U5VGD4

Product

Feature Type CDS
Coding Frame 1
Product Name
succinate dehydrogenase (B subunit)
Product Name Confidence: Class 2
Synonyms succinate dehydrogenase iron-sulfur protein
Evidence for Translation
Detected using LC-MS/MS (26.15 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.10
Kyte-Doolittle Hydrophobicity Value -0.212
Molecular Weight (kDa) 26.2
Isoelectric Point (pI) 6.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 526 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003779 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1584
Search term: sdhB

Human Homologs

Ensembl 110, assembly GRCh38.p14
succinate dehydrogenase complex iron sulfur subunit B [Source:HGNC Symbol;Acc:HGNC:10681]
E-value: 7.3e-70
Percent Identity: 56.5
Ensembl 110, assembly GRCh38.p14
succinate dehydrogenase complex iron sulfur subunit B [Source:HGNC Symbol;Acc:HGNC:10681]
E-value: 7.3e-70
Percent Identity: 56.5

References

Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12.
Darlison MG, Spencer ME, Guest JR
Eur J Biochem 1984 Jun 1;141(2):351-9
PubMed ID: 6376123
Regulation of succinate dehydrogenase (sdhCDAB) operon expression in Escherichia coli in response to carbon supply and anaerobiosis: role of ArcA and Fnr.
Park SJ, Tseng CP, Gunsalus RP
Mol Microbiol 1995 Feb;15(3):473-82
PubMed ID: 7783618
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344