Pseudomonas aeruginosa PAO1, PA1586 (sucB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1586
Name
sucB
Replicon chromosome
Genomic location 1727118 - 1728347 (+ strand)

Cross-References

RefSeq NP_250277.1
GI 15596783
Affymetrix PA1586_sucB_at
Entrez 881930
GenBank AAG04975.1
INSDC AAG04975.1
NCBI Locus Tag PA1586
protein_id(GenBank) gb|AAG04975.1|AE004586_13|gnl|PseudoCAP|PA1586
TIGR NTL03PA01587
UniParc UPI00000C53F5
UniProtKB Acc Q9I3D2
UniProtKB ID ODO2_PSEAE
UniRef100 UniRef100_Q9I3D2
UniRef50 UniRef50_P52993
UniRef90 UniRef90_Q9I3D2

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydrolipoamide succinyltransferase (E2 subunit)
Product Name Confidence: Class 2
Synonyms 2-oxoglutarate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (42.89 kDa, PMID:21751344)
Charge (pH 7) -6.78
Kyte-Doolittle Hydrophobicity Value 0.055
Molecular Weight (kDa) 42.9
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 513 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003777 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1586
Search term: sucB

Human Homologs

Ensembl 110, assembly GRCh38.p14
dihydrolipoamide S-succinyltransferase [Source:HGNC Symbol;Acc:HGNC:2911]
E-value: 1.8e-71
Percent Identity: 46.9

References

Solution structure of the lipoyl domain of the 2-oxoglutarate dehydrogenase complex from Azotobacter vinelandii.
Berg A, Vervoort J, de Kok A
J Mol Biol 1996 Aug 23;261(3):432-42
PubMed ID: 8780784
Three-dimensional structure of the lipoyl domain from the dihydrolipoyl succinyltransferase component of the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli.
Ricaud PM, Howard MJ, Roberts EL, Broadhurst RW, Perham RN
J Mol Biol 1996 Nov 22;264(1):179-90
PubMed ID: 8950276
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344