Pseudomonas aeruginosa PAO1, PA1589 (sucD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1589
Name
sucD
Replicon chromosome
Genomic location 1731347 - 1732234 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250280.1
GI 15596786
Affymetrix PA1589_sucD_at
Entrez 882039
GenBank AAG04978.1
INSDC CAA58871.1
NCBI Locus Tag PA1589
protein_id(GenBank) gb|AAG04978.1|AE004587_2|gnl|PseudoCAP|PA1589
TIGR NTL03PA01590
UniParc UPI00001361BA
UniProtKB Acc Q51567
UniProtKB ID SUCD_PSEAE
UniRef100 UniRef100_Q51567
UniRef50 UniRef50_P36967
UniRef90 UniRef90_Q51567

Product

Feature Type CDS
Coding Frame 1
Product Name
succinyl-CoA synthetase alpha chain
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (30.27 kDa, PMID:21751344)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Charge (pH 7) -3.01
Kyte-Doolittle Hydrophobicity Value 0.192
Molecular Weight (kDa) 30.3
Isoelectric Point (pI) 6.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 560 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003774 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1589
Search term: sucD

Human Homologs

Ensembl 110, assembly GRCh38.p14
succinate-CoA ligase GDP/ADP-forming subunit alpha [Source:HGNC Symbol;Acc:HGNC:11449]
E-value: 9.7e-104
Percent Identity: 67.0

References

Primary structure of the succinyl-CoA synthetase of Escherichia coli.
Buck D, Spencer ME, Guest JR
Biochemistry 1985 Oct 22;24(22):6245-52
PubMed ID: 3002435
The crystal structure of succinyl-CoA synthetase from Escherichia coli at 2.5-A resolution.
Wolodko WT, Fraser ME, James MN, Bridger WA
J Biol Chem 1994 Apr 8;269(14):10883-90
PubMed ID: 8144675
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Succinyl coenzyme A synthetase of Pseudomonas aeruginosa with a broad specificity for nucleoside triphosphate (NTP) synthesis modulates specificity for NTP synthesis by the 12-kilodalton form of nucleoside diphosphate kinase.
Kapatral V, Bina X, Chakrabarty AM
J Bacteriol 2000 Mar;182(5):1333-9
PubMed ID: 10671455