Pseudomonas aeruginosa PAO1, PA1687 (speE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1687
Name
speE
Replicon chromosome
Genomic location 1836367 - 1837227 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250378.1
GI 15596884
Affymetrix PA1687_speE_at
DNASU PaCD00006765
Entrez 883061
GenBank AAG05076.1
INSDC AAG05076.1
NCBI Locus Tag PA1687
protein_id(GenBank) gb|AAG05076.1|AE004596_2|gnl|PseudoCAP|PA1687
TIGR NTL03PA01688
UniParc UPI0000135DEF
UniProtKB Acc Q9X6R0
UniProtKB ID SPEE1_PSEAE
UniRef100 UniRef100_Q9X6R0
UniRef50 UniRef50_Q9X6R0
UniRef90 UniRef90_Q9X6R0

Product

Feature Type CDS
Coding Frame 1
Product Name
spermidine synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.44
Kyte-Doolittle Hydrophobicity Value -0.239
Molecular Weight (kDa) 32.2
Isoelectric Point (pI) 4.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 351 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003702 (713 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1687
Search term: speE

Human Homologs

Ensembl 110, assembly GRCh38.p14
spermidine synthase [Source:HGNC Symbol;Acc:HGNC:11296]
E-value: 1.5e-53
Percent Identity: 37.5

References

Spermidine synthase of Escherichia coli: localization of the speE gene.
Tabor CW, Tabor H, Xie QW
Proc Natl Acad Sci U S A 1986 Aug;83(16):6040-4
PubMed ID: 3526348
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994