Pseudomonas aeruginosa PAO1, PA1781 (nirB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1781
Name
nirB
Synonym: nasD
Replicon chromosome
Genomic location 1926176 - 1928626 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250472.1
GI 15596978
Affymetrix PA1781_nirB_at
Entrez 877714
GenBank AAG05170.1
INSDC AAG05170.1
NCBI Locus Tag PA1781
protein_id(GenBank) gb|AAG05170.1|AE004603_10|gnl|PseudoCAP|PA1781
TIGR NTL03PA01782
UniParc UPI00000C5494
UniProtKB Acc Q9I2W1
UniProtKB ID Q9I2W1_PSEAE
UniRef100 UniRef100_Q9I2W1
UniRef50 UniRef50_Q0BRC7
UniRef90 UniRef90_A0A024HFS3

Product

Feature Type CDS
Coding Frame 1
Product Name
assimilatory nitrite reductase large subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.72
Kyte-Doolittle Hydrophobicity Value -0.210
Molecular Weight (kDa) 89.7
Isoelectric Point (pI) 6.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 641 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003614 (505 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1781
Search term: nirB

Human Homologs

References

Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al.
Lin JT, Goldman BS, Stewart V
J Bacteriol 1993 Apr;175(8):2370-8
PubMed ID: 8468296
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
Ogawa K, Akagawa E, Yamane K, Sun ZW, LaCelle M, Zuber P, Nakano MM
J Bacteriol 1995 Mar;177(5):1409-13
PubMed ID: 7868621