Pseudomonas aeruginosa PAO1, PA1787 (acnB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1787
Name
acnB
Replicon chromosome
Genomic location 1935035 - 1937644 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250478.1
GI 15596984
Affymetrix PA1787_acnB_at
Entrez 879984
GenBank AAG05176.1
INSDC AAG05176.1
NCBI Locus Tag PA1787
protein_id(GenBank) gb|AAG05176.1|AE004604_6|gnl|PseudoCAP|PA1787
TIGR NTL03PA01788
UniParc UPI00000C549A
UniProtKB Acc Q9I2V5
UniProtKB ID ACNB_PSEAE
UniRef100 UniRef100_Q9I2V5
UniRef50 UniRef50_P36683
UniRef90 UniRef90_Q9I2V5

Product

Feature Type CDS
Coding Frame 1
Product Name
aconitate hydratase 2
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (93.63 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -20.15
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 93.6
Isoelectric Point (pI) 5.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003611 (559 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1787
Search term: acnB

References

Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli.
Gruer MJ, Guest JR
Microbiology (Reading, Engl.) 1994 Oct;140 ( Pt 10)
PubMed ID: 8000525
The second aconitase (AcnB) of Escherichia coli.
Bradbury AJ, Gruer MJ, Rudd KE, Guest JR
Microbiology (Reading, Engl.) 1996 Feb;142 ( Pt 2)
PubMed ID: 8932712
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344