Pseudomonas aeruginosa PAO1, PA1795 (cysS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1795
Name
cysS
Replicon chromosome
Genomic location 1944747 - 1946129 (+ strand)

Cross-References

RefSeq NP_250486.1
GI 15596992
Affymetrix PA1795_cysS_at
Entrez 880609
GenBank AAG05184.1
INSDC AAG05184.1
NCBI Locus Tag PA1795
protein_id(GenBank) gb|AAG05184.1|AE004605_6|gnl|PseudoCAP|PA1795
TIGR NTL03PA01796
UniParc UPI0000136362
UniProtKB Acc Q9I2U7
UniProtKB ID SYC_PSEAE
UniRef100 UniRef100_B7VB84
UniRef50 UniRef50_Q0A7N3
UniRef90 UniRef90_Q02KT6

Product

Feature Type CDS
Coding Frame 1
Product Name
cysteinyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms cysteine-tRNA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -12.06
Kyte-Doolittle Hydrophobicity Value -0.343
Molecular Weight (kDa) 51.2
Isoelectric Point (pI) 5.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 653 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003603 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1795
Search term: cysS

Human Homologs

Ensembl 110, assembly GRCh38.p14
cysteinyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:25695]
E-value: 2.3e-59
Percent Identity: 34.9

References

Cysteinyl-tRNA synthetase is a direct descendant of the first aminoacyl-tRNA synthetase.
Avalos J, Corrochano LM, Brenner S
FEBS Lett 1991 Jul 29;286(1-2):176-80
PubMed ID: 1864365
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994