Pseudomonas aeruginosa PAO1, PA1806 (fabI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1806
Name
fabI
Synonym: envM
Replicon chromosome
Genomic location 1961227 - 1962024 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250497.1
GI 15597003
Affymetrix PA1806_fabI_at
Entrez 878311
GenBank AAG05195.1
INSDC AAG05195.1
NCBI Locus Tag PA1806
protein_id(GenBank) gb|AAG05195.1|AE004607_1|gnl|PseudoCAP|PA1806
TIGR NTL03PA01807
UniParc UPI000012A481
UniProtKB Acc Q9ZFE4
UniProtKB ID FABI_PSEAE
UniRef100 UniRef100_Q9ZFE4
UniRef50 UniRef50_Q9ZFE4
UniRef90 UniRef90_Q9ZFE4

Product

Feature Type CDS
Coding Frame 1
Product Name
NADH-dependent enoyl-ACP reductase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.13
Kyte-Doolittle Hydrophobicity Value 0.028
Molecular Weight (kDa) 28.0
Isoelectric Point (pI) 5.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4NQZ OXIDOREDUCTASE 11/26/13 Crystal Structure of the Pseudomonas aeruginosa Enoyl-Acyl Carrier Protein Reductase (FabI) in apo form Pseudomonas aeruginosa 2.604 X-RAY DIFFRACTION 100.0
4NR0 OXIDOREDUCTASE 11/26/13 Crystal structure of the Pseudomonas aeruginosa Enoyl-Acyl Carrier Protein Reductase (FabI) in complex with NAD+ and triclosan Pseudomonas aeruginosa 1.799 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 625 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003591 (441 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1806
Search term: fabI

Human Homologs

References

Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli.
Bergler H, Wallner P, Ebeling A, Leitinger B, Fuchsbichler S, Aschauer H, Kollenz G, Högenauer G, Turnowsky F
J Biol Chem 1994 Feb 25;269(8):5493-6
PubMed ID: 8119879
Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis.
Hoang TT, Schweizer HP
J Bacteriol 1999 Sep;181(17):5489-97
PubMed ID: 10464225