Pseudomonas aeruginosa PAO1, PA1843 (metH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1843
Name
metH
Replicon chromosome
Genomic location 1999889 - 2003593 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250534.1
GI 15597040
Affymetrix PA1843_metH_at
Entrez 880631
GenBank AAG05232.1
INSDC AAG05232.1
NCBI Locus Tag PA1843
protein_id(GenBank) gb|AAG05232.1|AE004610_5|gnl|PseudoCAP|PA1843
TIGR NTL03PA01844
UniParc UPI00000C54C7
UniProtKB Acc Q9I2Q2
UniProtKB ID METH_PSEAE
UniRef100 UniRef100_Q9I2Q2
UniRef50 UniRef50_P13009
UniRef90 UniRef90_Q9I2Q2

Product

Feature Type CDS
Coding Frame 1
Product Name
methionine synthase
Product Name Confidence: Class 2
Synonyms 5-methyltetrahydrofolate--homocysteine S-methyltransferase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -53.05
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 135.1
Isoelectric Point (pI) 4.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 504 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003557 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1843
Search term: metH

Human Homologs

Ensembl 110, assembly GRCh38.p14
5-methyltetrahydrofolate-homocysteine methyltransferase [Source:HGNC Symbol;Acc:HGNC:7468]
E-value: 0.0e+00
Percent Identity: 54.5
Ensembl 110, assembly GRCh38.p14
5-methyltetrahydrofolate-homocysteine methyltransferase [Source:HGNC Symbol;Acc:HGNC:7468]
E-value: 0.0e+00
Percent Identity: 54.8
Ensembl 110, assembly GRCh38.p14
5-methyltetrahydrofolate-homocysteine methyltransferase [Source:HGNC Symbol;Acc:HGNC:7468]
E-value: 0.0e+00
Percent Identity: 52.3
Ensembl 110, assembly GRCh38.p14
5-methyltetrahydrofolate-homocysteine methyltransferase [Source:HGNC Symbol;Acc:HGNC:7468]
E-value: 0.0e+00
Percent Identity: 51.2
Ensembl 110, assembly GRCh38.p14
5-methyltetrahydrofolate-homocysteine methyltransferase [Source:HGNC Symbol;Acc:HGNC:7468]
E-value: 0.0e+00
Percent Identity: 52.4

References

Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain.
Banerjee RV, Johnston NL, Sobeski JK, Datta P, Matthews RG
J Biol Chem 1989 Aug 15;264(23):13888-95
PubMed ID: 2668277
Cobalamin-dependent methionine synthase.
Banerjee RV, Matthews RG
FASEB J 1990 Mar;4(5):1450-9
PubMed ID: 2407589
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994