Pseudomonas aeruginosa PAO1, PA1865

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1865
Name
Synonym: FAN1
Replicon chromosome
Genomic location 2024065 - 2025744 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250556.1
GI 15597062
Affymetrix PA1865_at
Entrez 877940
GenBank AAG05254.1
INSDC AAG05254.1
NCBI Locus Tag PA1865
protein_id(GenBank) gb|AAG05254.1|AE004612_8|gnl|PseudoCAP|PA1865
TIGR NTL03PA01866
UniParc UPI00000C54DD
UniProtKB Acc Q9I2N0
UniProtKB ID FAN1_PSEAE
UniRef100 UniRef100_Q9I2N0
UniRef50 UniRef50_Q9I2N0
UniRef90 UniRef90_Q9I2N0

Product

Feature Type CDS
Coding Frame 1
Product Name
Fanconi anemia-associated nuclease 1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -11.02
Kyte-Doolittle Hydrophobicity Value -0.288
Molecular Weight (kDa) 64.5
Isoelectric Point (pI) 5.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R89 Hydrolase/DNA 08/30/14 Crystal structure of paFAN1 - 5' flap DNA complex with Manganase Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 4.002 X-RAY DIFFRACTION 100.0
4R8A Hydrolase/DNA 08/30/14 Crystal structure of paFAN1 - 5' flap DNA complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.2 X-RAY DIFFRACTION 100.0
5Y7G HYDROLASE/DNA 08/17/17 Crystal structure of paFAN1 bound to 1nt 5'flap DNA with gap Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.4 X-RAY DIFFRACTION 100.0
5Z6W HYDROLASE/DNA 01/25/18 Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap with Manganese Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.2 X-RAY DIFFRACTION 100.0
5Y7Q HYDROLASE/DNA 08/17/17 Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 2.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 34 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003538 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1865

Human Homologs

Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3
Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3
Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3
Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3
Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3
Ensembl 110, assembly GRCh38.p14
FANCD2 and FANCI associated nuclease 1 [Source:HGNC Symbol;Acc:HGNC:29170]
E-value: 4.5e-17
Percent Identity: 32.3

References

Crystal structure of a Fanconi anemia-associated nuclease homolog bound to 5' flap DNA: basis of interstrand cross-link repair by FAN1.
Gwon GH, Kim Y, Liu Y, Watson AT, Jo A, Etheridge TJ, Yuan F, Zhang Y, Kim Y, Carr AM, Cho Y
Genes Dev 2014 Oct 15;28(20):2276-90
PubMed ID: 25319828