Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1871
|
Name |
lasA
|
Replicon | chromosome |
Genomic location | 2032695 - 2033951 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_250562.1 |
GI | 15597068 |
Affymetrix | PA1871_lasA_at |
Entrez | 878260 |
GenBank | AAG05260.1 |
INSDC | AAG05260.1 |
NCBI Locus Tag | PA1871 |
protein_id(GenBank) | gb|AAG05260.1|AE004613_5|gnl|PseudoCAP|PA1871 |
TIGR | NTL03PA01872 |
UniParc | UPI000012E22F |
UniProtKB Acc | P14789 |
UniProtKB ID | LASA_PSEAE |
UniRef100 | UniRef100_P14789 |
UniRef50 | UniRef50_P14789 |
UniRef90 | UniRef90_P14789 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
LasA protease precursor
|
Synonyms |
staphylolytic protease preproenzyme LasA |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (45.52 kDa, PMID:21751344)
|
Charge (pH 7) | 8.26 |
Kyte-Doolittle Hydrophobicity Value | -0.254 |
Molecular Weight (kDa) | 45.5 |
Isoelectric Point (pI) | 9.05 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3IT7 | HYDROLASE | 08/27/09 | Crystal Structure of the LasA virulence factor from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.14 | X-RAY DIFFRACTION | 100.0 |
3IT5 | HYDROLASE | 08/27/09 | Crystal Structure of the LasA Virulence Factor from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | VFDB : VF0088 |
Category | LasA protease |
Evidence |
|
Host Organism | |
Notes | |
Reference | 11333985 |
Source 2 | |
Database | PseudoCAP |
Category | LasA protease |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Oryctolagus cuniculus |
Notes | LasA mutation reduced invasion of rabbit corneal epithelial (RCE) cells by 77% |
Reference | 12904569 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003532 (299 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1871
Search term: lasA
Search term: LasA protease precursor
|
Activation of an elastase precursor by the lasA gene product of Pseudomonas aeruginosa.
Goldberg JB, Ohman DE
J Bacteriol 1987 Oct;169(10):4532-9
PubMed ID: 2820931
|
Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides.
Kessler E, Safrin M, Gustin JK, Ohman DE
J Biol Chem 1998 Nov 13;273(46):30225-31
PubMed ID: 9804780
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.
Olson JC, Ohman DE
J Bacteriol 1992 Jun;174(12):4140-7
PubMed ID: 1597429
|
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299
|