Pseudomonas aeruginosa PAO1, PA1871 (lasA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1871
Name
lasA
Replicon chromosome
Genomic location 2032695 - 2033951 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250562.1
GI 15597068
Affymetrix PA1871_lasA_at
Entrez 878260
GenBank AAG05260.1
INSDC AAG05260.1
NCBI Locus Tag PA1871
protein_id(GenBank) gb|AAG05260.1|AE004613_5|gnl|PseudoCAP|PA1871
TIGR NTL03PA01872
UniParc UPI000012E22F
UniProtKB Acc P14789
UniProtKB ID LASA_PSEAE
UniRef100 UniRef100_P14789
UniRef50 UniRef50_P14789
UniRef90 UniRef90_P14789

Product

Feature Type CDS
Coding Frame 1
Product Name
LasA protease precursor
Product Name Confidence: Class 1
Synonyms staphylolytic protease preproenzyme LasA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (45.52 kDa, PMID:21751344)
Charge (pH 7) 8.26
Kyte-Doolittle Hydrophobicity Value -0.254
Molecular Weight (kDa) 45.5
Isoelectric Point (pI) 9.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3IT7 HYDROLASE 08/27/09 Crystal Structure of the LasA virulence factor from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.14 X-RAY DIFFRACTION 100.0
3IT5 HYDROLASE 08/27/09 Crystal Structure of the LasA Virulence Factor from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0088
Category LasA protease
Evidence
Host Organism
Notes
Reference 11333985
Source 2
Database PseudoCAP
Category LasA protease
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Oryctolagus cuniculus
Notes LasA mutation reduced invasion of rabbit corneal epithelial (RCE) cells by 77%
Reference 12904569

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003532 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1871
Search term: lasA

Human Homologs

References

Activation of an elastase precursor by the lasA gene product of Pseudomonas aeruginosa.
Goldberg JB, Ohman DE
J Bacteriol 1987 Oct;169(10):4532-9
PubMed ID: 2820931
Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides.
Kessler E, Safrin M, Gustin JK, Ohman DE
J Biol Chem 1998 Nov 13;273(46):30225-31
PubMed ID: 9804780
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.
Olson JC, Ohman DE
J Bacteriol 1992 Jun;174(12):4140-7
PubMed ID: 1597429
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299