Pseudomonas aeruginosa PAO1, PA1886 (polB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1886
Name
polB
Synonym: dinA
Replicon chromosome
Genomic location 2054911 - 2057274 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250577.1
GI 15597083
Affymetrix PA1886_polB_at
Entrez 878669
GenBank AAG05275.1
INSDC AAG05275.1
NCBI Locus Tag PA1886
protein_id(GenBank) gb|AAG05275.1|AE004614_9|gnl|PseudoCAP|PA1886
TIGR NTL03PA01887
UniParc UPI00000C54EF
UniProtKB Acc Q9I2L1
UniProtKB ID Q9I2L1_PSEAE
UniRef100 UniRef100_Q9I2L1
UniRef50 UniRef50_P21189
UniRef90 UniRef90_Q9I2L1

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase II
Product Name Confidence: Class 2
Synonyms DNA-directed DNA polymerase II
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.97
Kyte-Doolittle Hydrophobicity Value -0.380
Molecular Weight (kDa) 89.7
Isoelectric Point (pI) 7.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 158 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003524 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1886
Search term: polB
Search term: DNA polymerase II

Human Homologs

Ensembl 110, assembly GRCh38.p14
DNA polymerase alpha 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9173]
E-value: 1.8e-32
Percent Identity: 25.5
Ensembl 110, assembly GRCh38.p14
DNA polymerase alpha 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9173]
E-value: 1.8e-32
Percent Identity: 25.5

References

DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.
Bonner CA, Hays S, McEntee K, Goodman MF
Proc Natl Acad Sci U S A 1990 Oct;87(19):7663-7
PubMed ID: 2217198
Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli.
Chen H, Sun Y, Stark T, Beattie W, Moses RE
DNA Cell Biol 1990 Nov;9(9):631-5
PubMed ID: 2261080
The Escherichia coli polB locus is identical to dinA, the structural gene for DNA polymerase II. Characterization of Pol II purified from a polB mutant.
Qiu Z, Goodman MF
J Biol Chem 1997 Mar 28;272(13):8611-7
PubMed ID: 9079692