Pseudomonas aeruginosa PAO1, PA1902 (phzD2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1902
Name
phzD2
Replicon chromosome
Genomic location 2072935 - 2073558 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250593.1
GI 15597099
Affymetrix PA1902_s_at
DNASU PaCD00006856
Entrez 881873
GenBank AAG05291.1
NCBI Locus Tag PA1902
protein_id(GenBank) gb|AAG05291.1|AE004616_5|gnl|PseudoCAP|PA1902
TIGR NTL03PA01903
UniProtKB Acc Q7DC80
UniProtKB ID Q7DC80_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
phenazine biosynthesis protein PhzD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.89
Kyte-Doolittle Hydrophobicity Value -0.094
Molecular Weight (kDa) 23.2
Isoelectric Point (pI) 5.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1NF9 HYDROLASE 12/13/02 Crystal Structure of PhzD protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0
1NF8 HYDROLASE 12/13/02 Crystal structure of PhzD protein active site mutant with substrate Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 99.5

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Isochorismic Acid DB02793 DrugBank 5.1.4 Q7DC80 Phenazine biosynthesis protein PhzD 100.0 207 3.6e-116
Formic acid DB01942 DrugBank 5.1.4 Q7DC80 Phenazine biosynthesis protein PhzD 100.0 207 3.6e-116

Virulence Evidence

Source 1
Database VFDB
Category Phenazine biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 231 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001494 (1079 members)
Putative Inparalogs 1 predicted inparalogs
phenazine biosynthesis protein PhzD (PA4213) 100.0% identity

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1902
Search term: phzD2

Human Homologs

References

Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
J Bacteriol 2001 Nov;183(21):6454-65
PubMed ID: 11591691
Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84.
Pierson LS 3rd, Gaffney T, Lam S, Gong F
FEMS Microbiol Lett 1995 Dec 15;134(2-3):299-307
PubMed ID: 8586283