Pseudomonas aeruginosa PAO1, PA1927 (metE)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1927
Name	metE
Replicon	chromosome
Genomic location	2106580 - 2108880 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_250617.1
GI	15597123
Affymetrix	PA1927_metE_at
Entrez	877662
GenBank	AAG05315.1
INSDC	AAG05315.1
NCBI Locus Tag	PA1927
protein_id(GenBank)	gb|AAG05315.1|AE004619_2|gnl|PseudoCAP|PA1927
TIGR	NTL03PA01927
UniParc	UPI000012EF7C
UniProtKB Acc	P57703
UniProtKB ID	METE_PSEAE
UniRef100	UniRef100_P57703
UniRef50	UniRef50_O50008
UniRef90	UniRef90_P57703

Product

Feature Type	CDS
Coding Frame	1
Product Name	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase Product Name Confidence: Class 2
Synonyms	cobalamin-independent methionine synthase
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-14.64
Kyte-Doolittle Hydrophobicity Value	-0.227
Molecular Weight (kDa)	86.2
Isoelectric Point (pI)	5.67

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	Victors : 4203
Category	Not available / Not known
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Rattus norvegicus
Notes
Infection model	Rat agar bead model of chronic lung infection
Reference	14641575

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 363 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003495 (526 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1927 Search term: metE Search term: 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase

Human Homologs

References

Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes. Maxon ME, Redfield B, Cai XY, Shoeman R, Fujita K, Fisher W, Stauffer G, Weissbach H, Brot N Proc Natl Acad Sci U S A 1989 Jan;86(1):85-9 PubMed ID: 2643109

Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem. González JC, Banerjee RV, Huang S, Sumner JS, Matthews RG Biochemistry 1992 Jul 7;31(26):6045-56 PubMed ID: 1339288