Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1982
|
Name |
exaA
|
Replicon | chromosome |
Genomic location | 2167281 - 2169152 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_250672.1 |
GI | 15597178 |
Affymetrix | PA1982_exaA_at |
Entrez | 880475 |
GenBank | AAG05370.1 |
INSDC | AAC79657.1 |
NCBI Locus Tag | PA1982 |
protein_id(GenBank) | gb|AAG05370.1|AE004624_9|gnl|PseudoCAP|PA1982 |
TIGR | NTL03PA01982 |
UniParc | UPI000012A32B |
UniProtKB Acc | Q9Z4J7 |
UniProtKB ID | EXAA_PSEAE |
UniRef100 | UniRef100_Q9Z4J7 |
UniRef50 | UniRef50_A8R3S4 |
UniRef90 | UniRef90_Q9Z4J7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
quinoprotein ethanol dehydrogenase
|
Synonyms |
quinoprotein alcohol dehydrogenase |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:6.6, molecular weight:68.1, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 0.77 |
Kyte-Doolittle Hydrophobicity Value | -0.463 |
Molecular Weight (kDa) | 68.1 |
Isoelectric Point (pI) | 7.11 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1FLG | OXIDOREDUCTASE | 08/14/00 | CRYSTAL STRUCTURE OF THE QUINOPROTEIN ETHANOL DEHYDROGENASE FROM PSEUDOMONAS AERUGINOSA | Pseudomonas aeruginosa | 2.6 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Pyrroloquinoline Quinone | DB03205 | DrugBank | 5.1.4 | Q9Z4J7 | Quinoprotein ethanol dehydrogenase | 100.0 | 623 | 0.0e+00 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 163 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003450 (426 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1982
Search term: exaA
Search term: quinoprotein ethanol dehydrogenase
|
Quaternary structure of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa and its reoxidation with a novel cytochrome c from this organism.
Schrover JM, Frank J, van Wielink JE, Duine JA
Biochem J 1993 Feb 15;290 ( Pt 1)(Pt 1):123-7
PubMed ID: 8382472
|
Characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of Comamonas testosteroni.
Stoorvogel J, Kraayveld DE, Van Sluis CA, Jongejan JA, De Vries S, Duine JA
Eur J Biochem 1996 Feb 1;235(3):690-8
PubMed ID: 8654419
|
Quinoprotein ethanol dehydrogenase of Pseudomonas aeruginosa is a homodimer--sequence of the gene and deduced structural properties of the enzyme.
Diehl A, von Wintzingerode F, Görisch H
Eur J Biochem 1998 Oct 15;257(2):409-19
PubMed ID: 9826187
|
A complex regulatory network controls aerobic ethanol oxidation in Pseudomonas aeruginosa: indication of four levels of sensor kinases and response regulators.
Mern DS, Ha SW, Khodaverdi V, Gliese N, Görisch H
Microbiology (Reading) 2010 May;156(Pt 5):1505-1516
PubMed ID: 20093290
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|