Pseudomonas aeruginosa PAO1, PA1982 (exaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1982
Name
exaA
Replicon chromosome
Genomic location 2167281 - 2169152 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250672.1
GI 15597178
Affymetrix PA1982_exaA_at
Entrez 880475
GenBank AAG05370.1
INSDC AAC79657.1
NCBI Locus Tag PA1982
protein_id(GenBank) gb|AAG05370.1|AE004624_9|gnl|PseudoCAP|PA1982
TIGR NTL03PA01982
UniParc UPI000012A32B
UniProtKB Acc Q9Z4J7
UniProtKB ID EXAA_PSEAE
UniRef100 UniRef100_Q9Z4J7
UniRef50 UniRef50_A8R3S4
UniRef90 UniRef90_Q9Z4J7

Product

Feature Type CDS
Coding Frame 1
Product Name
quinoprotein ethanol dehydrogenase
Product Name Confidence: Class 1
Synonyms quinoprotein alcohol dehydrogenase
Evidence for Translation
Detected by two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:6.6, molecular weight:68.1, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.77
Kyte-Doolittle Hydrophobicity Value -0.463
Molecular Weight (kDa) 68.1
Isoelectric Point (pI) 7.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1FLG OXIDOREDUCTASE 08/14/00 CRYSTAL STRUCTURE OF THE QUINOPROTEIN ETHANOL DEHYDROGENASE FROM PSEUDOMONAS AERUGINOSA Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Pyrroloquinoline Quinone DB03205 DrugBank 5.1.4 Q9Z4J7 Quinoprotein ethanol dehydrogenase 100.0 623 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 163 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003450 (426 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1982
Search term: exaA

Human Homologs

References

Quaternary structure of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa and its reoxidation with a novel cytochrome c from this organism.
Schrover JM, Frank J, van Wielink JE, Duine JA
Biochem J 1993 Feb 15;290 ( Pt 1)(Pt 1):123-7
PubMed ID: 8382472
Characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of Comamonas testosteroni.
Stoorvogel J, Kraayveld DE, Van Sluis CA, Jongejan JA, De Vries S, Duine JA
Eur J Biochem 1996 Feb 1;235(3):690-8
PubMed ID: 8654419
Quinoprotein ethanol dehydrogenase of Pseudomonas aeruginosa is a homodimer--sequence of the gene and deduced structural properties of the enzyme.
Diehl A, von Wintzingerode F, Görisch H
Eur J Biochem 1998 Oct 15;257(2):409-19
PubMed ID: 9826187
A complex regulatory network controls aerobic ethanol oxidation in Pseudomonas aeruginosa: indication of four levels of sensor kinases and response regulators.
Mern DS, Ha SW, Khodaverdi V, Gliese N, Görisch H
Microbiology (Reading) 2010 May;156(Pt 5):1505-1516
PubMed ID: 20093290
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802