Pseudomonas aeruginosa PAO1, PA2009 (hmgA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2009
Name
hmgA
Replicon chromosome
Genomic location 2197432 - 2198730 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250699.1
GI 15597205
Affymetrix PA2009_hmgA_at
Entrez 877745
GenBank AAG05397.1
INSDC AAD23385.1
NCBI Locus Tag PA2009
protein_id(GenBank) gb|AAG05397.1|AE004627_5|gnl|PseudoCAP|PA2009
TIGR NTL03PA02009
UniParc UPI000012C6CC
UniProtKB Acc Q9X4G0
UniProtKB ID HGD_PSEAE
UniRef100 UniRef100_B7VA96
UniRef50 UniRef50_Q88E47
UniRef90 UniRef90_B7VA96

Product

Feature Type CDS
Coding Frame 1
Product Name
homogentisate 1,2-dioxygenase
Product Name Confidence: Class 2
Synonyms homogentisicase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.01
Kyte-Doolittle Hydrophobicity Value -0.267
Molecular Weight (kDa) 47.8
Isoelectric Point (pI) 5.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 122 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003430 (505 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2009
Search term: hmgA

Human Homologs

Ensembl 110, assembly GRCh38.p14
homogentisate 1,2-dioxygenase [Source:HGNC Symbol;Acc:HGNC:4892]
E-value: 5.0e-125
Percent Identity: 52.9

References

Molecular characterization of a gene encoding a homogentisate dioxygenase from Aspergillus nidulans and identification of its human and plant homologues.
Fernández-Cañón JM, Peñalva MA
J Biol Chem 1995 Sep 8;270(36):21199-205
PubMed ID: 7673153
The molecular basis of alkaptonuria.
Fernández-Cañón JM, Granadino B, Beltrán-Valero de Bernabé D, Renedo M, Fernández-Ruiz E, Peñalva MA, Rodríguez de Córdoba S
Nat Genet 1996 Sep;14(1):19-24
PubMed ID: 8782815
The human homogentisate 1,2-dioxygenase (HGO) gene.
Granadino B, Beltrán-Valero de Bernabé D, Fernández-Cañón JM, Peñalva MA, Rodríguez de Córdoba S
Genomics 1997 Jul 15;43(2):115-22
PubMed ID: 9244427
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
Fuenmayor SL, Wild M, Boyes AL, Williams PA
J Bacteriol 1998 May;180(9):2522-30
PubMed ID: 9573207
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994