Pseudomonas aeruginosa PAO1, PA2023 (galU)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA2023
|
| Name |
galU
Synonym: hasC Synonym: gtaB |
| Replicon | chromosome |
| Genomic location | 2215102 - 2215941 (+ strand) |
| Transposon Mutants | 1 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_250713.1 |
| GI | 15597219 |
| Affymetrix | PA2023_galU_at |
| DNASU | PaCD00006903 |
| Entrez | 879968 |
| GenBank | AAG05411.1 |
| INSDC | AAG05411.1 |
| NCBI Locus Tag | PA2023 |
| protein_id(GenBank) | gb|AAG05411.1|AE004628_9|gnl|PseudoCAP|PA2023 |
| TIGR | NTL03PA02023 |
| UniParc | UPI000012B1B3 |
| UniProtKB Acc | Q9I291 |
| UniProtKB ID | GALU_PSEAE |
| UniRef100 | UniRef100_Q9I291 |
| UniRef50 | UniRef50_Q9I291 |
| UniRef90 | UniRef90_Q9I291 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
UTP--glucose-1-phosphate uridylyltransferase
|
| Synonyms |
glucose-1-phosphate uridylyltransferase UDP-glucose pyrophosphorylase |
| Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (31.24 kDa, PMID:21751344)
|
| Charge (pH 7) | -6.25 |
| Kyte-Doolittle Hydrophobicity Value | -0.211 |
| Molecular Weight (kDa) | 31.2 |
| Isoelectric Point (pI) | 5.12 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
Virulence Evidence
| Source 1 | |
| Database | PseudoCAP |
| Category | LPS core region biosynthesis |
| Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
| Host Organism | Mus musculus |
| Notes | |
| Infection model | murine corneal infection. |
| Infection model | acute mouse pulmonary Infection model |
| Reference | 15213167 |
| Source 2 | |
| Database | Victors : 4241 |
| Category | LPS core region biosynthesis |
| Evidence |
ECO:0000015
mutant phenotype evidence
|
| Host Organism | Mus musculus |
| Notes | |
| Reference | 15213167 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 622 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG003418 (530 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA2023
Search term: galU
Search term: UTP--glucose-1-phosphate uridylyltransferase
|
Human Homologs
References
|
Molecular characterization of hasC from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose pyrophosphorylase activity.
Crater DL, Dougherty BA, van de Rijn I
J Biol Chem 1995 Dec 1;270(48):28676-80
PubMed ID: 7499387
|
|
The gene encoding UDP-glucose pyrophosphorylase is required for the synthesis of xanthan gum in Xanthomonas campestris.
Wei CL, Lin NT, Weng SF, Tseng YH
Biochem Biophys Res Commun 1996 Sep 24;226(3):607-12
PubMed ID: 8831665
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|