Pseudomonas aeruginosa PAO1, PA2247 (bkdA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2247
Name
bkdA1
Replicon chromosome
Genomic location 2473213 - 2474445 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250937.1
GI 15597443
Affymetrix PA2247_bkdA1_at
Entrez 877901
GenBank AAG05635.1
INSDC AAG05635.1
NCBI Locus Tag PA2247
protein_id(GenBank) gb|AAG05635.1|AE004650_6|gnl|PseudoCAP|PA2247
TIGR NTL03PA02247
UniParc UPI00000D74B0
UniProtKB Acc Q9I1M2
UniProtKB ID ODBA_PSEAE
UniRef100 UniRef100_Q9I1M2
UniRef50 UniRef50_P09060
UniRef90 UniRef90_Q9I1M2

Product

Feature Type CDS
Coding Frame 1
Product Name
2-oxoisovalerate dehydrogenase (alpha subunit)
Product Name Confidence: Class 2
Synonyms branched-chain alpha-keto acid decarboxylase
3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.25
Kyte-Doolittle Hydrophobicity Value -0.286
Molecular Weight (kDa) 45.3
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 421 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002213 (788 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2247
Search term: bkdA1

Human Homologs

References

Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.
Sykes PJ, Burns G, Menard J, Hatter K, Sokatch JR
J Bacteriol 1987 Apr;169(4):1619-25
PubMed ID: 3549697
Similarity of the E1 subunits of branched-chain-oxoacid dehydrogenase from Pseudomonas putida to the corresponding subunits of mammalian branched-chain-oxoacid and pyruvate dehydrogenases.
Burns G, Brown T, Hatter K, Idriss JM, Sokatch JR
Eur J Biochem 1988 Sep 15;176(2):311-7
PubMed ID: 3416875
Transcriptional analysis of the promoter region of the Pseudomonas putida branched-chain keto acid dehydrogenase operon.
Madhusudhan KT, Huang G, Burns G, Sokatch JR
J Bacteriol 1990 Oct;172(10):5655-63
PubMed ID: 2211503