Pseudomonas aeruginosa PAO1, PA2249 (bkdB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2249
Name
bkdB
Replicon chromosome
Genomic location 2475495 - 2476781 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250939.1
GI 15597445
Affymetrix PA2249_bkdB_at
Entrez 880679
GenBank AAG05637.1
INSDC AAG05637.1
NCBI Locus Tag PA2249
protein_id(GenBank) gb|AAG05637.1|AE004650_8|gnl|PseudoCAP|PA2249
TIGR NTL03PA02249
UniParc UPI00000D74B2
UniProtKB Acc Q9I1M0
UniProtKB ID ODB2_PSEAE
UniRef100 UniRef100_Q9I1M0
UniRef50 UniRef50_Q9I1M0
UniRef90 UniRef90_Q9I1M0

Product

Feature Type CDS
Coding Frame 1
Product Name
branched-chain alpha-keto acid dehydrogenase (lipoamide component)
Product Name Confidence: Class 2
Synonyms dihydrolipoamide branched chain transacylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.33
Kyte-Doolittle Hydrophobicity Value -0.138
Molecular Weight (kDa) 45.8
Isoelectric Point (pI) 6.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 513 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003240 (459 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2249
Search term: bkdB

Human Homologs

Ensembl 110, assembly GRCh38.p14
dihydrolipoamide branched chain transacylase E2 [Source:HGNC Symbol;Acc:HGNC:2698]
E-value: 1.5e-44
Percent Identity: 30.5

References

Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.
Sykes PJ, Burns G, Menard J, Hatter K, Sokatch JR
J Bacteriol 1987 Apr;169(4):1619-25
PubMed ID: 3549697
Comparison of the amino acid sequences of the transacylase components of branched chain oxoacid dehydrogenase of Pseudomonas putida, and the pyruvate and 2-oxoglutarate dehydrogenases of Escherichia coli.
Burns G, Brown T, Hatter K, Sokatch JR
Eur J Biochem 1988 Sep 1;176(1):165-9
PubMed ID: 3046941