Pseudomonas aeruginosa PAO1, PA2445 (gcvP2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2445
Name
gcvP2
Replicon chromosome
Genomic location 2743800 - 2746679 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251135.1
GI 15597641
Affymetrix PA2445_gcvP2_at
Entrez 882927
GenBank AAG05833.1
INSDC AAG05833.1
NCBI Locus Tag PA2445
protein_id(GenBank) gb|AAG05833.1|AE004672_1|gnl|PseudoCAP|PA2445
TIGR NTL03PA02445
UniParc UPI00000C56D8
UniProtKB Acc Q9I137
UniProtKB ID GCSP1_PSEAE
UniRef100 UniRef100_Q9I137
UniRef50 UniRef50_P23378
UniRef90 UniRef90_Q9I137

Product

Feature Type CDS
Coding Frame 1
Product Name
glycine cleavage system protein P2
Product Name Confidence: Class 2
Synonyms glycine decarboxylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -18.76
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 103.9
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 474 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003093 (925 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2445
Search term: gcvP2

Human Homologs

Ensembl 110, assembly GRCh38.p14
glycine decarboxylase [Source:HGNC Symbol;Acc:HGNC:4313]
E-value: 1.1e-310
Percent Identity: 55.9

References

Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system.
Okamura-Ikeda K, Ohmura Y, Fujiwara K, Motokawa Y
Eur J Biochem 1993 Sep 1;216(2):539-48
PubMed ID: 8375392
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994