Pseudomonas aeruginosa PAO1, PA2476 (dsbG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2476
Name
dsbG
Replicon chromosome
Genomic location 2794206 - 2794976 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentYu, Liang, et al. A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria.Science Bulletin(2021). https://doi.org/10.1016/j.scib.2021.03.003.

Cross-References

RefSeq NP_251166.1
GI 15597672
Affymetrix PA2476_dsbG_at
Entrez 882549
GenBank AAG05864.1
INSDC AAG05864.1
NCBI Locus Tag PA2476
protein_id(GenBank) gb|AAG05864.1|AE004675_5|gnl|PseudoCAP|PA2476
TIGR NTL03PA02476
UniParc UPI00001298EE
UniProtKB Acc Q9I106
UniProtKB ID DSBG_PSEAE
UniRef100 UniRef100_Q9I106
UniRef50 UniRef50_Q9I106
UniRef90 UniRef90_Q9I106

Product

Feature Type CDS
Coding Frame 1
Product Name
thiol:disulfide interchange protein DsbG
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 0.14
Kyte-Doolittle Hydrophobicity Value -0.279
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 93 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003077 (477 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2476
Search term: dsbG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A new Escherichia coli gene, dsbG, encodes a periplasmic protein involved in disulphide bond formation, required for recycling DsbA/DsbB and DsbC redox proteins.
Andersen CL, Matthey-Dupraz A, Missiakas D, Raina S
Mol Microbiol 1997 Oct;26(1):121-32
PubMed ID: 9383195
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994