Pseudomonas aeruginosa PAO1, PA2570 (lecA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2570
Name
lecA
Synonym: lecA (changed from pa1L)
Synonym: pa1L
Replicon chromosome
Genomic location 2905182 - 2905550 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251260.1
GI 15597766
Affymetrix PA2570_pa1L_at
DNASU PaCD00007110
Entrez 882335
GenBank AAG05958.1
INSDC AAG05958.1
NCBI Locus Tag PA2570
protein_id(GenBank) gb|AAG05958.1|AE004685_4|gnl|PseudoCAP|PA2570
TIGR NTL03PA02570
UniParc UPI0000165EC5
UniProtKB Acc Q05097
UniProtKB ID PA1L_PSEAE
UniRef100 UniRef100_Q05097
UniRef50 UniRef50_Q05097
UniRef90 UniRef90_Q05097

Product

Feature Type CDS
Coding Frame 1
Product Name
LecA
Product Name Confidence: Class 1
Synonyms PA-I galactophilic lectin
LecA (changed from PA-I galactophilic lectin)
Evidence for Translation
Charge (pH 7) -2.60
Kyte-Doolittle Hydrophobicity Value -0.148
Molecular Weight (kDa) 12.9
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4YW7 SUGAR BINDING PROTEIN 03/20/15 Structural Insight into Divalent Galactoside Binding to Pseudomonas aeruginosa lectin LecA Pseudomonas aeruginosa 1.82 X-RAY DIFFRACTION 100.0
3ZYB SUGAR BINDING PROTEIN 08/19/11 CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH GALAG0 AT 2.3 A RESOLUTION Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 2.29 X-RAY DIFFRACTION 100.0
3ZYH SUGAR BINDING PROTEIN/INHIBITOR 08/23/11 CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH GALBG0 AT 1.5 A RESOLUTION Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.501 X-RAY DIFFRACTION 100.0
4LJH SUGAR BINDING PROTEIN 07/04/13 Crystal Structure of Pseudomonas aeruginosa Lectin LecA Complexed with 1-Methyl-3-indolyl-b-D-galactopyranoside at 1.45 A Resolution Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 100.0
3ZYF SUGAR BINDING PROTEIN 08/22/11 CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH NPG AT 1.9 A RESOLUTION PSEUDOMONAS AERUGINOSA 1.944 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 4 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003000 (296 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2570
Search term: lecA
Search term: LecA

Human Homologs

References

The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa.
Diggle SP, Stacey RE, Dodd C, Cámara M, Williams P, Winzer K
Environ Microbiol 2006 Jun;8(6):1095-104
PubMed ID: 16689730
On the specificity of the D-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of D-galactose and thiogalactose.
Garber N, Guempel U, Belz A, Gilboa-Garber N, Doyle RJ
Biochim Biophys Acta 1992 Jun 12;1116(3):331-3
PubMed ID: 1610890
Analysis of the amino acid sequence of the Pseudomonas aeruginosa galactophilic PA-I lectin.
Avichezer D, Katcoff DJ, Garber NC, Gilboa-Garber N
J Biol Chem 1992 Nov 15;267(32):23023-7
PubMed ID: 1429650
Pseudomonas aeruginosa PA-I lectin gene molecular analysis and expression in Escherichia coli.
Avichezer D, Gilboa-Garber N, Garber NC, Katcoff DJ
Biochim Biophys Acta 1994 May 17;1218(1):11-20
PubMed ID: 8193158
PA-I and PA-II lectin interactions with the ABO(H) and P blood group glycosphingolipid antigens may contribute to the broad spectrum adherence of Pseudomonas aeruginosa to human tissues in secondary infections.
Gilboa-Garber N, Sudakevitz D, Sheffi M, Sela R, Levene C
Glycoconj J 1994 Oct;11(5):414-7
PubMed ID: 7696845