Pseudomonas aeruginosa PAO1, PA2612 (serS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2612
Name
serS
Replicon chromosome
Genomic location 2953416 - 2954696 (- strand)

Cross-References

RefSeq NP_251302.1
GI 15597808
Affymetrix PA2612_serS_at
Entrez 882318
GenBank AAG06000.1
INSDC AAG06000.1
NCBI Locus Tag PA2612
protein_id(GenBank) gb|AAG06000.1|AE004689_12|gnl|PseudoCAP|PA2612
TIGR NTL03PA02612
UniParc UPI00000C5769
UniProtKB Acc Q9I0M6
UniProtKB ID SYS_PSEAE
UniRef100 UniRef100_B7UW10
UniRef50 UniRef50_Q6F8G5
UniRef90 UniRef90_A4VLU7

Product

Feature Type CDS
Coding Frame 1
Product Name
seryl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -9.93
Kyte-Doolittle Hydrophobicity Value -0.356
Molecular Weight (kDa) 47.2
Isoelectric Point (pI) 5.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6HE3 LIGASE 08/20/18 Pseudomonas aeruginosa Seryl-tRNA Synthetase in Complex with 5'-O-(N-(L-seryl)-sulfamoyl)cytidine Pseudomonas aeruginosa 2.16 X-RAY DIFFRACTION 99.8
6HDZ LIGASE 08/20/18 Pseudomonas aeruginosa Seryl-tRNA Synthetase in Complex with 5'-O-(N-(L-seryl)-Sulfamoyl)uridine Pseudomonas aeruginosa 2.06 X-RAY DIFFRACTION 99.8
6HE1 LIGASE 08/20/18 Pseudomonas aeruginosa Seryl-tRNA Synthetase in Complex with 5'-O-(N-(L-seryl)-sulfamoyl)N3-methyluridine Pseudomonas aeruginosa 2.22 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 651 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002939 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2612
Search term: serS

Human Homologs

Ensembl 110, assembly GRCh38.p14
seryl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:10537]
E-value: 1.5e-52
Percent Identity: 32.2

References

A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 A.
Cusack S, Berthet-Colominas C, Härtlein M, Nassar N, Leberman R
Nature 1990 Sep 20;347(6290):249-55
PubMed ID: 2205803
Seryl-tRNA synthetase from Escherichia coli: functional evidence for cross-dimer tRNA binding during aminoacylation.
Vincent C, Borel F, Willison JC, Leberman R, Härtlein M
Nucleic Acids Res 1995 Apr 11;23(7):1113-8
PubMed ID: 7537870
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994