Pseudomonas aeruginosa PAO1, PA2634 (aceA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: aceA
Replicon chromosome
Genomic location 2977756 - 2979351 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Commentinduced by acetate, citronellol and leucine, and repressed by growth on glucose or citrate


RefSeq NP_251324.1
GI 15597830
Affymetrix PA2634_at
Entrez 882341
GenBank AAG06022.1
INSDC AAG06022.1
NCBI Locus Tag PA2634
protein_id(GenBank) gb|AAG06022.1|AE004692_1|gnl|PseudoCAP|PA2634
UniParc UPI00000C577B
UniProtKB Acc Q9I0K4
UniRef100 UniRef100_Q9I0K4
UniRef50 UniRef50_O50078
UniRef90 UniRef90_Q9I0K4


Feature Type CDS
Coding Frame 1
Product Name
isocitrate lyase AceA
Product Name Confidence: Class 1
Synonyms isocitrate lyase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.85
Kyte-Doolittle Hydrophobicity Value -0.317
Molecular Weight (kDa) 58.9
Isoelectric Point (pI) 5.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6G1O LYASE 03/21/18 Structure of Pseudomonas aeruginosa Isocitrate Lyase, ICL PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.882 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category isocitrate lyase
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes isocitrate lyase mutant is attenuated for virulence in rat agar bead model of chronic lung infection
Infection model rat agar bead model of chronic lung infection
Reference 18524916

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 273 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002919 (359 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2634
Search term: aceA


Identification of the aceA gene encoding isocitrate lyase required for the growth of Pseudomonas aeruginosa on acetate, acyclic terpenes and leucine.
Díaz-Pérez AL, Román-Doval C, Díaz-Pérez C, Cervantes C, Sosa-Aguirre CR, López-Meza JE, Campos-García J
FEMS Microbiol. Lett. 2007 Apr;269(2):309-16
PubMed ID: 17319879
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994